Low dose radiation-induced adaptive response preventing HPRT mutation is Fhit independent
Low dose radiation-induced adaptive response preventing HPRT mutation is Fhit independent
复制标题
低剂量辐射诱导的预防 HPRT 突变的适应性反应与 Fhit 无关。
DOI：
10.1080/09553000902883828
复制
发表时间：
2009-01-01
影响因子：
2.6
通讯作者：
Wang, Ya
中科院分区：
文献类型：
--
作者：
Lu, Lin;Hu, Baocheng;Wang, Ya
目的：研究脆性组氨酸三联体（Fhit）是否可以预防IR诱导的次黄嘌呤鸟嘌呤磷酸核糖转移酶（HPRT）突变，以及Fhit是否通过低剂量诱导的适应性反应在预防HPRT突变中发挥作用。材料和方法：通过制备单独表达血凝素（HA）或HA-Fhit融合蛋白的构建体并将载体转染至HeLa细胞，建立具有或不具有Fhit表达的人类细胞系。通过观察不同剂量 IR 后建立的细胞系中 HPRT 突变率，检查 Fhit 对电离辐射 (IR) 诱导的突变的影响。通过观察暴露于 0.1 Gy 并随后进行高剂量 IR 或紫外线 (UV) 暴露的已建立细胞系中的 HPRT 突变率，检查 Fhit 对低剂量 IR 诱导的适应性反应的作用。结果：低剂量（0.1 Gy）不影响这些细胞系中的 HPRT 突变率。 Fhit 可以防止高剂量 IR (2 Gy) 诱导的突变，就像它可以防止 UV 诱导的突变一样。然而，低剂量的 IR (0.1 Gy) 诱导的适应性反应可防止高剂量的 IR 和 UV 诱导的突变，无论是否有 Fhit 表达的细胞。结论：Fhit 可以预防 IR 诱导的 HPRT 突变，并且通过低剂量 IR 诱导的适应性反应来预防突变与 Fhit 无关。
Purpose: To study whether fragile histidine triad (Fhit) prevents IR-induced hypoxanthineguanine phosphoribosyltransferase (HPRT) mutation and whether Fhit plays any role in preventing HPRT mutation through low dose-induced adaptive response. Materials and methods: Establishing human cell lines with or without Fhit expression by making constructs expressing hemagglutinin (HA) alone or HA-Fhit fusion protein and transfecting the vector to HeLa cells. The effects of Fhit on ionising radiation (IR)-induced mutation were examined by observing HPRT mutation rates in the established cell lines following different doses of IR. The role of Fhit on low dose IR-induced adaptive response were examined by observing HPRT mutation rates in the established cell lines that were exposed to 0.1 Gy and followed with high dose IR or ultraviolet (UV) exposure. Results: Low dose (0.1 Gy) does not affect HPRT mutation rates in these cell lines. Fhit prevents high dose IR (2 Gy)-induced mutation as it prevents UV-induced mutation. However, low dose of IR (0.1 Gy)-induced adaptive response prevents both high doses of IR and UV-induced mutation in both the cells with and without Fhit expression. Conclusions: Fhit prevents IR-induced HPRT mutation and preventing mutation through low dose of IR-induced adaptive response is Fhit independent.