Synergistic inhibition of csal1 and csal3 in granulosa cell proliferation and steroidogenesis of hen ovarian prehierarchical development
Synergistic inhibition of csal1 and csal3 in granulosa cell proliferation and steroidogenesis of hen ovarian prehierarchical development
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csal1和csal3对母鸡卵巢分级前发育的颗粒细胞增殖和类固醇生成的协同抑制作用
DOI：
10.1093/biolre/ioz137
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发表时间：
2019-07
影响因子：
3.6
通讯作者：
Mishra Birendra
中科院分区：
文献类型：
--
作者：
Zhu Hongyan;Qin Ning;Xu Xiaoxing;Sun Xue;Chen Xiaoxia;Zhao Jinghua;Xu Rifu;Mishra Birendra
摘要SALL 1和SALL 3是一类转录因子，在许多物种的发育和器官形成过程中起着重要的调控作用。然而，SALL 1和SALL 3在鸡前级卵泡发育中的功能作用尚不清楚。本研究旨在探讨csal 1和csal 3在鸡卵巢颗粒细胞增殖、分化和前级卵泡选择中的作用及其机制。我们的数据表明，csal 1和csal 3 transcription高表达的颗粒细胞的前分级卵泡，其蛋白主要定位于颗粒细胞和卵母细胞的细胞质，以及在卵巢基质和上皮细胞。初步揭示了csal 1和csal 3可能通过易位机制参与鸡前级卵泡的发育。此外，我们的研究结果表明，丰富的CCND 1，Bcat，星星，CYP 11 A1，和FSHR的mRNA在颗粒细胞，和颗粒细胞的增殖水平从prehierarchical卵泡显着增加通过siRNA介导的敲低csal 1或/和csal 3。相反，颗粒细胞中Csal 1或/和Csal 3的过表达导致其显著减少。此外，csal 1和csal 3一起发挥的调节作用比csal 1或csal 3中的任何一个都强得多。这些结果表明，csal 1和csal 3发挥协同抑制作用的颗粒细胞增殖，分化，和类固醇激素的生成在前分级卵泡体外发育。本研究结果为进一步研究csal 1和csal 3在体内调控鸡卵巢前级卵泡发育和生长的分子机制奠定了基础。转录因子csal 1和csal 3可能在PF发育过程中对颗粒细胞增殖、分化和类固醇合成起协同抑制作用。
Abstract SALL1 and SALL3 are transcription factors that play an essential role in regulating developmental processes and organogenesis in many species. However, the functional role of SALL1 and SALL3 in chicken prehierarchical follicle development is unknown. This study aimed to explore the potential role and mechanism of csal1 and csal3 in granulosa cell proliferation, differentiation, and follicle selection within the prehierarchical follicles of hen ovary. Our data demonstrated that the csal1 and csal3 transcriptions were highly expressed in granulosa cells of prehierarchical follicles, and their proteins were mainly localized in the cytoplasm of granulosa cells and oocytes as well as in the ovarian stroma and epithelium. It initially revealed that both csal1 and csal3 may be involved in chicken prehierarchical follicle development via a translocation mechanism. Furthermore, our results showed an abundance of CCND1, Bcat, StAR, CYP11A1, and FSHR mRNA in granulosa cells, and the proliferation levels of granulosa cells from the prehierarchical follicles were significantly increased by siRNA-mediated knockdown of csal1 or/and csal3. Conversely, the overexpression of csal1 or/and csal3 in the granulosa cells led to a remarkably decreased of them. Moreover, csal1 and csal3 together exert a much stronger effect on the regulation than any of csal1 or csal3. These results indicated that csal1 and csal3 play synergistic inhibitory roles on granulosa cell proliferation, differentiation, and steroidogenesis during prehierarchical follicle development in vitro. The current data provide a basis of molecular mechanisms of csal1 and csal3 in controlling the prehierarchical follicle development and growth of hen ovary in vivo. Summary Sentence The transcription factors csal1 and csal3 might play a synergistic inhibitory role on granulosa cell proliferation, differentiation, and steroidogenesis during PF development.
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SLIT3-ROBO1/ROBO2在母鸡卵巢分级前卵泡颗粒细胞增殖、分化和卵泡选择中的意义
影响因子：
3.9
作者：
Rifu Xu;Ning Qin;Xiaoxing Xu;Xue Sun;Xiaoxia Chen;Jinghua Zhao
通讯作者：
Jinghua Zhao
影响因子：
3.8
作者：
Miao F;Zhang X;Cao Y;Wang Y;Zhang X
通讯作者：
Zhang X
DOI：
10.12659/msm.897301
发表时间：
2016-09-23
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Xiang Y;Song Y;Li Y;Zhao D;Ma L;Tan L
通讯作者：
Tan L
影响因子：
3.7
作者：
Tanwar PS;Zhang L;Kaneko-Tarui T;Curley MD;Taketo MM;Rani P;Roberts DJ;Teixeira JM
通讯作者：
Teixeira JM
影响因子：
6.6
作者：
E Hermosilla V;Salgado G;Riffo E;Escobar D;Hepp MI;Farkas C;Galindo M;Morín V;García-Robles MA;Castro AF;Pincheira R
通讯作者：
Pincheira R