Affinity of IgE antibody to antigen influences allergen-induced histamine release
Affinity of IgE antibody to antigen influences allergen-induced histamine release
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DOI：
10.1046/j.1365-2222.2000.00921.x
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发表时间：
2000-11-01
影响因子：
6.1
通讯作者：
Akiyama, K
中科院分区：
文献类型：
--
作者：
Mita, H;Yasueda, H;Akiyama, K
背景虽然抗体对抗原的亲和力被认为是决定其生物学效应的重要因素，目的研究IgE抗体与Der p2的亲和力对Der p2诱导的人嗜碱性粒细胞组胺释放的影响。结果14例Der p 2敏感的哮喘患者，其IgE抗体的最可能Kd值为5.6 ~ 177.8pM。在Der p2诱导的组胺释放中，Der p2特异性IgE抗体的Kd值与过敏原的敏感性呈显著正相关（r（s）= 0.797，P = 0.0040），提示特异性IgE抗体的亲和力越高，释放特定水平组胺所需的变应原量越低。组胺释放的敏感性与IgE抗体对其抗原的亲和力密切相关。
Background Although affinity of an antibody for an antigen is recognized to be an important factor in determining its biological effects, little is known about the relevance of such affinity of IgE antibodies to the functional response.Objectives To investigate the effect of IgE antibody affinity to Der p 2 on Der p 2-induced histamine release from human basophils.Methods The most probable value of the dissociation constant (Kd) of IgE antibody to Der p 2 was calculated and histamine release by Der p 2-challenged leucocytes was used to evaluate the biological efficacy of the IgE antibody.Results The most probable Kd value of IgE antibody to Der p 2 ranged from 5.6 to 177.8 pM in 14 asthmatic patients sensitive to Der p 2. A significant correlation was observed in Der p 2-induced histamine release between the sensitivity and the Kd value for Der p 2-specific IgE antibody (r(s) = 0.797, P = 0.0040), suggesting that the higher the affinity, the lower the amount of allergen required for the release of a specific level of histamine.Conclusion Apart from the changes associated with the reactivity, the sensitivity of histamine release is closely related to the affinity of IgE antibody for its antigen.