Fertility of mice receiving vitrified adult mouse ovaries
Fertility of mice receiving vitrified adult mouse ovaries
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DOI：
10.1530/rep.1.01030
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发表时间：
2006-04-01
期刊：
影响因子：
3.8
通讯作者：
Jishage, K
中科院分区：
文献类型：
--
作者：
Hani, T;Tachibe, T;Jishage, K
卵巢冷冻保存是一种保存实验动物生殖细胞的有效技术，由于雌性创始人不育或线粒体DNA发生突变，需要保存雌性生殖细胞。虽然它是可能的冷冻保存未成熟的小鼠卵巢与高度的活力，玻璃化与几种冷冻保护剂的混合物，冷冻保存的成年小鼠卵巢的活力仍然是未知的。在这里，我们调查了小鼠卵巢的活力在不同年龄冷冻保存后，玻璃化技术。从10天、4周、10周和7个月大的雌性未经产的绿色荧光蛋白（GFP）转基因小鼠中收集供体卵巢，并通过玻璃化冷冻保存。将玻璃化温热的卵巢原位移植到4或10周龄的小鼠体内。所有实验组均获得GFP阳性幼仔。在4周龄受体中，移植10日龄、4周龄、10周龄和7月龄供体卵巢后，GFP阳性幼仔数分别占总幼仔数的44%、9%、12%和4%。在10周龄受体中，GFP阳性的幼仔占移植10日龄、4周龄、10周龄和7月龄供体卵巢的幼仔总数的比例分别为36%、16%、2%和9%。此外，GFP阳性的幼崽也获得了从受体移植卵巢的供体没有正常的发情周期。我们的研究结果表明，小鼠卵巢的玻璃化冷冻保存是一种有用的方法，从不同年龄的小鼠的雌性生殖细胞的保存。
Cryopreservation of the ovaries is a useful technology for preservation of germ cells from experimental animals, because of the female founder is infertile or has mutated mitochondrial DNA, preservation of female germ cells is necessary. Although it is possible to cryopreserve immature mouse ovaries with a high degree of viability by vitrification with a mixture of several cryoprotectants, the viability of cryopreserved adult mouse ovaries is still unknown. Here, we investigated the viability of mouse ovaries at various ages after cryopreservation by vitrification techniques. Donor ovaries were collected from 10-day-, 4-week-, 10-week- and 7-month-old, female, nulliparous, green fluorescence protein (GFP)-transgenic mice and cryopreserved by vitrification. The vitrified-warmed ovaries were orthotopically transplanted to 4- or 10-week-old mice. G FP-positive pups were obtained in all experimental groups. In the 4-week-old recipients, the percentages of GFP-positive pups among the total pups from recipients transplanted with ovaries of 10-day-, 4-week-, 10-week- and 7-month-old donors were 44%, 9%, 12% and 4% respectively. In the 10-week-old recipients, the percentages of GFP-positive pups among the total pups from recipients transplanted with ovaries of 10-day-, 4-week-, 10-week- and 7-month-old donors were 36%, 16%, 2 % and 9% respectively. Furthermore, GFP-positive pups also were obtained from recipients transplanted with ovaries of donors without normal estrous cyclicity. Our results indicate that cryopreservation of mouse ovaries by vitrification is a useful method for the preservation of female germ cells from mice of various ages.