Differential ability of two simian virus 40 strains to induce malignancies in weanling hamsters.
Differential ability of two simian virus 40 strains to induce malignancies in weanling hamsters.
复制标题
两种猿猴病毒 40 株诱导断奶仓鼠恶性肿瘤的能力差异。
DOI：
10.1016/j.virol.2004.09.024
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Butel,JanetS
中科院分区：
文献类型：
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作者：
Vilchez,RegisA;Brayton,CoryF;Wong,Connie;Zanwar,Preeti;Killen,DeannaE;Jorgensen,JeffreyL;Butel,JanetS
猴病毒40（SV 40）的不同毒株存在并与一些人类恶性肿瘤相关，但尚不清楚SV 40毒株在体内的生物学潜力是否不同。在两个长期实验中，21日龄的叙利亚金仓鼠通过腹腔内途径接种两种不同的SV 40菌株（107空斑形成单位/动物），并随访8或12个月。比较了对最初从猴肾细胞中分离的菌株VA 45 -54和从人癌症中回收的菌株SVCPC的体内反应。用细胞培养基腹膜内接种相同年龄的对照动物。仅在感染SV 40的动物中发生恶性肿瘤，而在对照组中未发生。在8个月（11/22，50% vs. 4/20，20%）和12个月（7/15，47% vs. 3/13，23%）的实验中，SVCPC株感染动物的肿瘤发生率高于VA 45 -54。组织学上，肿瘤类似于间皮瘤、骨肉瘤和低分化肉瘤。两种病毒株均发生肺和淋巴结转移。免疫组化检测到T抗原在大多数肿瘤细胞中表达。抗T抗原抗体产生的几乎所有的荷瘤动物和约三分之二的那些没有发展肿瘤病毒接种后。SV 40病毒中和抗体在所有荷瘤动物中检测到，分别在92%和38%的SVCPC和VA 45 -54接种的动物中检测到，这些动物没有发生肿瘤。抗体滴度通常在有肿瘤的动物中高于没有肿瘤的动物。对照组动物未产生病毒抗体。从15个测试的肿瘤中的2个中回收感染性病毒。本研究表明，这两株SV 40毒株在生物学上存在差异，影响正常宿主感染后的转归，包括恶性肿瘤的发生和中和抗体的产生，并证实了不同进化枝的SV 40毒株在体内生物学特性存在差异的原理。
Different strains of simian virus 40 (SV40) exist and are associated with some human malignancies, but it is not known if SV40 strains differ in biological potential in vivo. In two long-term experiments, Syrian golden hamsters 21 days of age were inoculated by the intraperitoneal route with two different strains of SV40 (107plaque-forming units/animal) and were followed for 8 or 12 months. In vivo responses to strain VA45–54, isolated originally from monkey kidney cells, and to strain SVCPC, recovered from human cancers, were compared. Control animals of the same age were inoculated intraperitoneally with cell culture media. Malignancies developed only in animals infected with SV40 and not in controls. The rate of tumor development was more frequent among animals infected with strain SVCPC than with VA45–54, both in experiments held for 8 months (11/22, 50% vs. 4/20, 20%) and for 12 months (7/15, 47% vs. 3/13, 23%). Histologically, the tumors resembled mesotheliomas, osteosarcoma, and poorly differentiated sarcomas. Metastases to lung and lymph nodes occurred with both viral strains. T-antigen expression was detected in most tumor cells by immunohistochemistry. Anti-T-antigen antibodies were produced by almost all tumor-bearing animals and by about two-thirds of those that did not develop tumors after virus inoculation. SV40 viral neutralizing antibodies were detected in all tumor-bearing animals and in 92% and 38% of those inoculated with SVCPC and VA45–54, respectively, that failed to develop tumors. Antibody titers were usually higher in animals with tumors than in those without. Control animals did not develop viral antibodies. Infectious virus was recovered from 2 of 15 tumors tested. This study showed that there are biological differences between these two SV40 strains that influence the outcome of infections in normal hosts, including the development of malignancies and neutralizing antibody, and proved the principle that SV40 strains from different clades can vary in biological properties in vivo.