Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson's disease-related symptoms.
Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson's disease-related symptoms.
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Pikuni-Blackfeet 传统医学：用于治疗帕金森病相关症状的药用植物的神经保护活性。
DOI：
10.1016/j.jep.2017.01.001
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发表时间：
2017
影响因子：
5.4
通讯作者：
Rochet,Jean-Christophe
中科院分区：
文献类型：
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作者：
deRusJacquet,Aurélie;Tambe,MitaliArun;Ma,SinYing;McCabe,GeorgeP;Vest,JayHansfordC;Rochet,Jean-Christophe
帕金森病（PD）是一种多因素的神经退行性疾病，影响5%的85岁以上人群。目前的治疗主要涉及多巴胺替代疗法，这导致运动症状的暂时缓解，但未能减缓潜在的神经变性。因此，需要具有神经保护活性的安全PD疗法。在这项研究中，我们分析了来自蒙大拿州西部的Pikuni-Blackfeet部落成员用于治疗PD相关症状的当代草药实践，在努力确定药用植物，是负担得起的传统社区和获得更大的人口。研究的目的这项研究的目的是（i）确定药用植物使用的皮库尼-黑脚部落治疗与PD或其他中枢神经系统疾病有关的症状的个人，以及（ii）在PD的细胞模型中，根据抗氧化和神经保护活性来表征所鉴定的植物的子集。材料和方法对治疗者和当地人进行了采访，印第安保留地。收集植物样品，并产生水提取物用于随后的分析。一个子集的植物提取物进行了测试的能力，以诱导激活的NRF 2介导的转录反应，并防止神经毒性引起的PD相关毒素鱼藤酮和paraquat.ResultsThe民族药理学的采访导致的文件中的26种药用植物用于治疗各种疾病和疾病，包括与PD相关的症状。七种植物提取物（共测试10种提取物）显示在原代皮质星形胶质细胞中激活Nrf 2介导的转录活性。提取物准备从大蒜丁香，红三叶pratenseflowers，和Amelanchier树木表现出神经保护活性对毒性引起的鱼藤酮，而只有提取物准备从大蒜和Amelanchier树木减轻PQ诱导的多巴胺能细胞death.ConclusionsOur的研究结果突出了潜在的临床效用的植物用于药用的几代人的皮库尼黑脚的人，他们揭示了植物提取物减缓帕金森病神经变性的机制。
Ethnopharmacological relevanceParkinson's disease (PD) is a multifactorial neurodegenerative disorder affecting 5% of the population over the age of 85 years. Current treatments primarily involve dopamine replacement therapy, which leads to temporary relief of motor symptoms but fails to slow the underlying neurodegeneration. Thus, there is a need for safe PD therapies with neuroprotective activity. In this study, we analyzed contemporary herbal medicinal practices used by members of the Pikuni-Blackfeet tribe from Western Montana to treat PD-related symptoms, in an effort to identify medicinal plants that are affordable to traditional communities and accessible to larger populations.Aim of the studyThe aims of this study were to (i) identify medicinal plants used by the Pikuni-Blackfeet tribe to treat individuals with symptoms related to PD or other CNS disorders, and (ii) characterize a subset of the identified plants in terms of antioxidant and neuroprotective activities in cellular models of PD.Materials and methodsInterviews of healers and local people were carried out on the Blackfeet Indian reservation. Plant samples were collected, and water extracts were produced for subsequent analysis. A subset of botanical extracts was tested for the ability to induce activation of the Nrf2-mediated transcriptional response and to protect against neurotoxicity elicited by the PD-related toxins rotenone and paraquat.ResultsThe ethnopharmacological interviews resulted in the documentation of 26 medicinal plants used to treat various ailments and diseases, including symptoms related to PD. Seven botanical extracts (out of a total of 10 extracts tested) showed activation of Nrf2-mediated transcriptional activity in primary cortical astrocytes. Extracts prepared fromAllium sativumcloves,Trifolium pratenseflowers, andAmelanchier arboreaberries exhibited neuroprotective activity against toxicity elicited by rotenone, whereas only the extracts prepared fromAllium sativumandAmelanchier arboreaalleviated PQ-induced dopaminergic cell death.ConclusionsOur findings highlight the potential clinical utility of plants used for medicinal purposes over generations by the Pikuni-Blackfeet people, and they shed light on mechanisms by which the plant extracts could slow neurodegeneration in PD.