SPERM BINDING CHARACTERISTICS OF MURINE ZONA PELLUCIDA
SPERM BINDING CHARACTERISTICS OF MURINE ZONA PELLUCIDA
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DOI：
10.1095/biolreprod13.3.340
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发表时间：
1975-01-01
影响因子：
3.6
通讯作者：
WOLF, DP
中科院分区：
文献类型：
--
作者：
INOUE, M;WOLF, DP
建立了研究小鼠透明带精子结合特性的实验条件。新鲜附睾精子与未受精卵带的结合是一个时间依赖性过程，反映了体外孵化条件下精子获能的发生。相比之下，大量获能精子与未受精卵子带的结合发生与时间因素无关。获能精子与未受精卵透明带（每个透明带 > 100 个）和体内产生的 2 细胞胚胎（每个透明带 0-5 个）的结合存在明显差异，为评估透明带反应的发生提供了基础。在类似的条件下，获能的精子大量结合到体外产生的2细胞胚胎的透明带上。已经讨论了体外受精卵中透明带反应不正常或不充分的可能原因。
Experimental conditions were established for an investigation of the sperm binding characteristics of the mouse zona pellucida. Fresh epididymal sperm binding to zonae of unfertilized eggs was a time-dependent process, reflecting the occurrence of sperm capacitation underin vitroincubation conditions. In contrast, capacitated sperm binding in large numbers to zonae of unfertilized ova occurred independently of temporal considerations. A dramatic difference was apparent in the binding of capacitated sperm to zonae of unfertilized eggs (>100 per zona) and toin vivoproduced 2-cell embryos (0–5 per zona), providing a basis for assessing the occurrence of a zona reaction. Under comparable conditions, capacitated sperm bound in large numbers to zonae ofin vitroproduced 2-cell embryos. Possible reasons for a subnormal or inadequate zona reaction in eggs inseminatedin vitrohave been discussed.