Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults
Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults
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DOI：
10.1016/s0304-3940(02)00199-4
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发表时间：
2002-05-24
影响因子：
2.5
通讯作者：
Nishimoto, I
中科院分区：
文献类型：
--
作者：
Tajima, H;Niikura, T;Nishimoto, I
An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
