Magnetic motor evoked potential monitoring in the rat
Magnetic motor evoked potential monitoring in the rat
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DOI：
10.3171/spi.1999.91.2.0205
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发表时间：
1999-10-01
影响因子：
4.1
通讯作者：
Shields, CB
中科院分区：
文献类型：
--
作者：
Linden, RD;Zhang, YP;Shields, CB
物体。作者进行了一项研究，旨在为大鼠下行运动通路提供客观的电生理学评估。可能成为脊髓损伤研究中预测预后的一种手段。在清醒、非麻醉、束缚的大鼠上记录不同条件下的经颅磁运动诱发电位(TMMEPs)。收集标准数据以确定模型的重复性，并评估改变刺激强度对诱发信号的影响。此外，还进行了一项实验，以确定产生的TMMEP是否是磁刺激时外壳内铜线圈运动产生的声音引起的听觉惊吓反应(ASR)电位的结果。磁刺激后诱发经颅磁运动诱发电位。在100%刺激强度下，前肢起始潜伏期平均为4.2+/-0.39毫秒，波幅为9.16+/-。3.44 mV。后肢起始潜伏期为6.5±0.47毫秒，波幅为11.47±5.25 mV。随着刺激强度的降低，TMMEP的起始潜伏期延长，反应幅度降低。ASR潜伏期比TMMEP潜伏期长，波幅小，变异性大。这些实验证明，在清醒的、非麻醉的大鼠身上可以记录到TMMEPs。诱发的信号易于诱发和重现。本文介绍一种无创监测大鼠脊髓生理完整性的新技术--TMMEPs。
Object. The authors conducted a study to provide an objective electrophysiological assessment of descending motor pathways in rats. which may become a means for predicting outcome in spinal cord injury research.Methods. Transcranial magnetic motor evoked potentials (TMMEPs) were recorded under various conditions in awake, nonanesthetized, restrained rats. Normative data were collected to determine the reproducibility of the model and to evaluate the effect of changing the stimulus intensity on the evoked signals. In addition, an experiment was per formed to determine if the TMMEPs produced were the result of auditory startle response (ASR) potentials elicited by the sound generated by the movement of the copper coil inside its casing during magnetic stimulation.Transcranial magnetic motor evoked potentials were elicited after magnetic stimulation. At 100% stimulus intensity, the mean forelimb onset latency was 4.2 +/- 0.39 msec, and the amplitude was 9.16 +/-. 3.44 mV. The hindlimb onset latency was 6.5 +/- 0.47 msec, and the amplitude was 11.47 +/- 5.25 mV. As the stimulus intensity was decreased, the TMMEP onset latency increased and the response amplitude decreased. The ASR potentials were shown to have longer latencies, smaller amplitudes, and were more variable than those of the TMMEPs.Conclusions. These experiments demonstrate that TMMEPs can be recorded in awake, nonanesthetized rats. The evoked signals were easy to elicit and reproduce. This paper introduces noninvasive TMMEPs as a new technique for monitoring the physiological integrity of the rat spinal cord.