Community participation in biofilm matrix assembly and function
Community participation in biofilm matrix assembly and function
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DOI：
10.1073/pnas.1421437112
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发表时间：
2015-03-31
影响因子：
11.1
通讯作者：
Andes, David R.
中科院分区：
文献类型：
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作者：
Mitchell, Kaitlin F.;Zarnowski, Robert;Andes, David R.
真菌白色念珠菌的生物膜产生细胞外基质，赋予诸如粘附和耐药性的性质。我们先前的研究表明，基质是复杂的，主要多糖成分是α-甘露聚糖、β-1，6葡聚糖和β-1，3葡聚糖。在这里，我们实施遗传，生物化学和药理学的方法来解开这三种成分的矩阵结构和功能的贡献。任何一种多糖成分的合成或输出的干扰改变了其他每种多糖的基质浓度。每一个这些也需要基质功能，如通过测定抗真菌药物氟康唑的螯合。这些结果表明，基质生物发生需要协调交付的个别基质多糖。为了了解协调是否发生在细胞水平或群落水平，我们询问是否可以通过生物膜共培养诱导基质缺陷突变株产生功能性基质。我们观察到，混合生物膜接种突变体含有中断在每个多糖途径恢复成熟的基质结构，组成和生物膜耐药性。我们的研究结果认为，功能性基质生物合成细胞外协调，从而反映了生物膜社区的合作行动。
Biofilms of the fungus Candida albicans produce extracellular matrix that confers such properties as adherence and drug resistance. Our prior studies indicate that the matrix is complex, with major polysaccharide constituents being alpha-mannan, beta-1,6 glucan, and beta-1,3 glucan. Here we implement genetic, biochemical, and pharmacological approaches to unravel the contributions of these three constituents to matrix structure and function. Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides. Each of these was also required for matrix function, as assessed by assays for sequestration of the antifungal drug fluconazole. These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides. To understand whether coordination occurs at the cellular level or the community level, we asked whether matrix-defective mutant strains could be coaxed to produce functional matrix through biofilm coculture. We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance. Our results argue that functional matrix biogenesis is coordinated extracellularly and thus reflects the cooperative actions of the biofilm community.