Interactions between sustained contractile activity and beta-adrenergic receptors in regulation of gene expression in skeletal muscles.
Interactions between sustained contractile activity and beta-adrenergic receptors in regulation of gene expression in skeletal muscles.
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持续收缩活动和β-肾上腺素受体之间的相互作用在骨骼肌基因表达调节中的作用。
DOI：
10.1152/ajpcell.1989.256.3.c506
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Williams,RS
中科院分区：
文献类型：
--
作者：
Kraus,WE;Bernard,TS;Williams,RS
连续电刺激支配成年家兔前室肌肉的运动神经10-21天，使β-肾上腺素能受体（β-AR）的密度和组织中3 '，5'-环磷酸腺苷（cAMP）的浓度增加2 - 3倍。cAMP和β-AR的变化与刺激诱导的线粒体酶比活性适应（增加2- 6倍）以及线粒体RNA、β-F1 ATP酶mRNA和肌红蛋白mRNA稳态浓度的变化（增加2- 11倍）平行发生。这些增加肌肉cAMP，β-AR，和蛋白质和mRNA产物的基因编码的蛋白质的氧化代谢发生在动物接受高剂量的普萘洛尔在电刺激期间。与编码氧化代谢蛋白质的基因相反，糖酵解酶醛缩酶A表达的活性诱导变化的方向和时间过程似乎与肌肉cAMP的变化无关;在cAMP最大上升之前的早期时间点，醛缩酶A mRNA的稳态浓度抑制最大（对照的20-25%）。此外，普萘洛尔的管理衰减的抑制作用的连续收缩活动的醛缩酶A的表达，即使在没有这种药物对cAMP在刺激肌肉的影响。我们的结论是，活动诱导的变化cAMP，β-AR，并在编码蛋白质的氧化代谢的重要基因的表达发生作为收缩活动的直接后果，并不需要伴随刺激β-AR。（250字处删节）
Continuous electrical stimulation for 10-21 days of the motor nerve innervating the anterior compartment muscles of adult rabbits increased both the density of beta-adrenergic receptors (beta-AR) and tissue concentrations of adenosine 3',5'-cyclic monophosphate (cAMP) by two to threefold. Changes in cAMP and in beta-AR occurred in parallel with stimulation-induced adaptations in the specific activity of mitochondrial enzymes (2- to 6-fold increases) and with changes in steady-state concentrations of mitochondrial RNA, beta-F1ATPase mRNA, and myoglobin mRNA (2- to 11-fold increases). These increases in muscle cAMP, in beta-AR, and in expression of protein and mRNA products of genes encoding proteins of oxidative metabolism occurred even in animals receiving high doses of propranolol during the period of electrical stimulation. In contrast to genes that encode proteins of oxidative metabolism, the direction and the time course of activity-induced changes in expression of the glycolytic enzyme aldolase A appeared to be unrelated to changes in muscle cAMP; suppression of steady-state concentrations of aldolase A mRNA was maximal (20-25% of control) at early time points preceding the maximal rise in cAMP. In addition, administration of propranolol attenuated the suppressive effect of continuous contractile activity on expression of aldolase A, even in the absence of an effect of this drug on cAMP in stimulated muscles. We conclude that activity-induced changes in cAMP, in beta-AR, and in expression of genes that encode proteins important for oxidative metabolism occur as a direct consequence of contractile activity and do not require concomitant stimulation of beta-AR.(ABSTRACT TRUNCATED AT 250 WORDS)