Quantitative Magnetic Resonance Imaging Assessment of Matrix Development in Cell-Seeded Natural Urinary Bladder Smooth Muscle Tissue-Engineered Constructs
Quantitative Magnetic Resonance Imaging Assessment of Matrix Development in Cell-Seeded Natural Urinary Bladder Smooth Muscle Tissue-Engineered Constructs
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DOI：
10.1089/ten.tec.2009.0099
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发表时间：
2010-08-01
影响因子：
3
通讯作者：
Farhat, Walid A.
中科院分区：
文献类型：
--
作者：
Cheng, Hai-Ling Margaret;Islam, Syed S.;Farhat, Walid A.
细胞种子天然支架的方法为复杂的软组织器官如膀胱和心脏的组织工程提供了巨大的希望。然而，对于细胞-自然支架相互作用或它们对磁共振成像（MRI）表征的影响知之甚少，这对于无创监测是有价值的。理想情况下，MRI除了提供组织结构和功能信息外，还应提供组织生化信息。在本研究中，在播种后7天的不同时间点，对对照和平滑肌细胞播种的天然膀胱基质进行定量MRI检查。MR弛豫时间（T1和T2）和扩散系数(D)的测量显示了与细胞存在不相容的总体变化。多组分T2提供了更大的特异性，揭示了短T2部分的时间变化，这与从种子细胞释放的胶原酶中生化测定的基质降解一致。这些基质变化是第一次被注意到，与合成材料相比，它们相对较早的发生可能是软组织基质所特有的。更重要的是，它们在组织学上不明显，但在定量MRI上显示。我们得出结论，定量MRI可以提供细胞-基质相互作用的具体信息，是一种很有前途的无创方法来了解和监测细胞种子天然支架再生。
The approach of cell-seeded natural scaffolds holds great promise for tissue engineering complicated soft-tissue organs such as the urinary bladder and heart. However, relatively little is known about cell-natural scaffold interactions or their influence on magnetic resonance imaging (MRI) characterization, which is valuable for noninvasive monitoring. Ideally, MRI should provide information on tissue biochemistry in addition to structure and function. In this study, quantitative MRI was performed on control and smooth muscle cell-seeded natural bladder matrices at different time points up to 7 days postseeding. Measurements of MR relaxation times (T1 and T2) and diffusion coefficient (D) showed an overall change that was incompatible with cell presence. Multicomponent T2 provided greater specificity, revealing time-course changes in the short T2 fraction that were consistent with biochemically determined matrix degradation from collagenase released from seeded cells. These matrix alterations are noted for the first time, and their relatively early occurrence may be unique to soft-tissue matrices compared with synthetic materials. More importantly, they are not evident on histology but are revealed on quantitative MRI. We conclude that quantitative MRI may provide specific information on cell-matrix interaction and is a promising noninvasive approach to understand and monitor cell-seeded natural scaffold-based regeneration.