Real-Time Photoluminescent Biosensing Based on Graphene Oxide-Coated Microplates: A Rapid Pathogen Detection Platform
Real-Time Photoluminescent Biosensing Based on Graphene Oxide-Coated Microplates: A Rapid Pathogen Detection Platform
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DOI：
10.1021/acs.analchem.0c02200
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发表时间：
2020-09-01
影响因子：
7.4
通讯作者：
Morales-Narvaez, Eden
中科院分区：
文献类型：
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作者：
Avila-Huerta, Mariana D.;Ortiz-Riano, Edwin J.;Morales-Narvaez, Eden
致病菌污染是对安全、人类健康和生态系统的重大威胁。在此，我们报告了一种优势的单步，免洗，实时细菌检测平台，只需一种抗体。大肠杆菌被检测为模型分析物。这项技术是基于氧化石墨烯涂层微孔板（GOMs）和光致发光生物探针（PLBs）。一方面，利用非辐射能量转移，GOMs被认为可以使那些没有通过抗体-细菌膜亲和力进行免疫反应的plb的光致发光失活。另一方面，那些进行免疫反应的PLBs保持其光致发光是因为(i)复合物（PLBs-细菌）与GOMs之间的距离和（ii）相同复合物与GOMs之间的亲和力较低。结果表明，该细菌检测平台具有快速和高灵敏度，检测限为2 CFU mL(-1)，最佳分析时间约为30 min。工业真实样品也成功地分析了广泛使用的格式，是适合于高通量的应用。此外，所提出的技术具有高度的变革性，因为氧化石墨烯能够淬灭不同的荧光团，并且可以通过简单地改变特定抗体来检测其他分析物。
Pathogenic bacterial contamination is a major threat to safety, human health, and ecosystems. Herein, we report an advantageous single-step, wash-free, and real-time bacterial detection platform operating with a single antibody. Escherichia col was detected as a model analyte. This technology is based on graphene oxide-coated microplates (GOMs) and photoluminescent bioprobes (PLBs). On the one hand, using nonradiative energy transfer, GOMs are conceived to deactivate the photoluminescence of those PLBs that are not experimenting immunoreactions via antibody-bacterial membrane affinity. On the other hand, those PLBs experimenting immunoreactions preserve their photoluminescence because of both (i) the distance between the complex (PLBs-bacteria) and GOMs and (ii) the low affinity between the same complex and GOMs. With an optimal analytical performance of similar to 30 min, the resulting bacterial detection platform was demonstrated to be fast and highly sensitive, exhibiting a limit of detection of similar to 2 CFU mL(-1). Industrial real samples were also successfully analyzed in a widely used format that is amenable to high-throughput applications. Moreover, the proposed technology is highly transformative, as graphene oxide is able to quench different fluorophores, and other analytes can be detected by simply changing the specific antibody.