DIPHTHERIA TOXIN SUBUNIT ACTIVE IN VITRO
DIPHTHERIA TOXIN SUBUNIT ACTIVE IN VITRO
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DOI：
10.1126/science.164.3884.1179
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发表时间：
1969-01-01
期刊：
影响因子：
56.9
通讯作者：
COLE, HA
中科院分区：
文献类型：
--
作者：
COLLIER, RJ;COLE, HA
白喉毒素暴露于二硫苏糖醇（和类似的硫醇）导致在烟酰胺腺嘌呤二核苷酸存在下，在催化哺乳动物氨酰转移酶II的腺苷二磷酸化中具有活性的亚基。同时，总ADP-核糖基化活性显著增加。在部分纯化的毒素制剂中检测到一种分子，该分子在大小和活性上与衍生亚基明显相同。
Exposure of diphtheria toxin to dithiothreitol (and similar thiols) resulted in a subunit which was active in catalyzing the adenosine diphosphateribosylation of mammalian aminoacyl-transferase II in the presence of nicotinamide adenine dinucleotide. At the same time there was a marked increase in total ADP-ribosylation activity. A molecule which was apparently identical to the derived subunit in size and activity was detected in partially purified preparations of toxin.