A novel protein Depp, which is induced by progesterone in human endometrial stromal cells activates Elk-1 transcription factor
A novel protein Depp, which is induced by progesterone in human endometrial stromal cells activates Elk-1 transcription factor
复制标题
DOI：
10.1093/molehr/gah186
复制
发表时间：
2005-07-01
影响因子：
4
通讯作者：
Fujita, J
中科院分区：
文献类型：
--
作者：
Watanabe, H;Nonoguchi, K;Fujita, J
子宫内膜基质细胞 (ESC) 的蜕膜化被认为是受到孕酮和/或 cAMP 的刺激，对于胚胎植入和胎盘形成至关重要。在这项研究中，我们从富含孕酮诱导基因的人类 ESC cDNA 文库中分离出编码孕酮 (Depp) 诱导的蜕膜蛋白的新克隆。 Depp mRNA 在多种人体组织中表达，包括胎盘、卵巢和肾脏。在分泌中期和晚期以及妊娠第一个月的蜕膜期间，在子宫内膜中观察到 Depp 表达增加。在体外，黄体酮治疗后 30 分钟内，Depp mRNA 在 ESC 中被诱导，但被抗孕激素 RU486 抑制。单独的雄激素也诱导德普表达。 Depp 增加了人胚胎肾 293 细胞中磷酸化 Erk 的水平并激活了 Elk-1 转录因子，这表明 Depp 通过影响基因表达来调节蜕膜化过程中和蜕膜中黄体酮的作用。阐明Depp在子宫内膜中的生物学功能将有助于我们了解蜕膜化和胎盘发育的分子机制。
Decidualization of the endometrial stromal cells (ESC), considered to be stimulated by progesterone and/or cAMP, is crucial for embryo implantation and placentation. In this study, we isolated a novel clone encoding decidual protein induced by progesterone ( Depp) from a human ESC cDNA library enriched with progesterone-inducible genes. Depp mRNA was expressed in various human tissues including placenta, ovary and kidney. Increased expression of Depp was observed in endometria during mid-and late-secretory phases and 1st trimester deciduas. In vitro, Depp mRNA was induced in ESC within 30 min of progesterone treatment, which was inhibited by the antiprogestin RU486. Androgen alone also induced Depp expression. Depp increased the level of phosphorylated Erk and activated the Elk-1 transcription factor in human embryonal kidney 293 cells, suggesting that Depp modulates the effects of progesterone during decidualization and in the decidua by affecting gene expression. Elucidation of the biological function of Depp in the endometrium will facilitate our understanding of the molecular mechanisms of decidualization and placental development.