Ischemic cerebral tissue response to subventricular zone cell transplantation measured by iterative self-organizing data analysis technique algorithm
Ischemic cerebral tissue response to subventricular zone cell transplantation measured by iterative self-organizing data analysis technique algorithm
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DOI：
10.1038/sj.jcbfm.9600288
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发表时间：
2006-11-01
影响因子：
6.3
通讯作者：
Chopp, Michael
中科院分区：
文献类型：
--
作者：
Li, Lian;Jiang, Quan;Chopp, Michael
为了研究SVZ细胞移植后大鼠脑缺血病变的变化及移植细胞对血管新生形态的影响，我们在栓塞性脑卒中发生48 h后，采用超顺磁标记方法将SVZ细胞内移植到大鼠脑内。在中风后约24小时、72小时和每周（n = 3）对所有接受（n = 8）和未接受（n = 3）细胞移植的动物获得一套完整的磁共振（MR）图像。通过高分辨率三维梯度回波图像跟踪移植细胞，并通过T-1、T-2和T-1sat图计算的ISODATA（迭代自组织数据分析技术算法）检测细胞与缺血性病变之间的相互作用。来自ISODATA的组织状态由一个特定的特征来表征，它代表了特征空间中与正常组织的偏差。移植的SVZ细胞在移植后1周内选择性地向大鼠脑缺血侧迁移，并接近病变边界。与对照组相比，细胞处理大鼠的平均病变大小显著减小（P < 0.05）。细胞移植诱导的组织特征的显著减少（P < 0.001）局限于移植细胞的位置，这些部位表现出稳定恢复的脑血流量（CBF）（约为正常CBF的85%）。血管新生存在于与移植物细胞相邻或周围的部位。我们的数据表明，map-ISODATA准确和动态地表征了缺血性病变及其对细胞治疗的反应。
To investigate the changes of the ischemic lesion in rat brain after subventricular zone (SVZ) cell transplantation and the influence of the grafted cells on the appearance of angiogenesis, SVZ cells, superparamagnetically labeled, were intracisternally transplanted into the rat brain 48 h after onset of embolic stroke. A complete set of magnetic resonance (MR) images was acquired for all animals with (n = 8) and without (n = 3) cell grafting at approximately 24 h, 72 h, and weekly for 6 weeks after stroke. Transplanted cells were tracked by high-resolution three-dimensional gradient-echo images and the interaction between the cells and ischemic lesion was detected by ISODATA (Iterative Self-Organizing Data Analysis Technique Algorithm) calculated from T-1, T-2 and T-1sat maps. Tissue status from ISODATA was characterized by a specific signature, which represents the deviation from normal tissue in the feature space. Transplanted SVZ cells selectively migrated towards the ischemic side of the rat brain and approached the lesion boundary within 1-week after grafting. Cell treated rats exhibited a significant reduction of average lesion size compared with control rats ( P < 0.05). A significant reduction of tissue signature (P < 0.001) induced by cell transplantation was localized to the position of grafted cells, and these sites exhibited stably restored cerebral blood flow (CBF) (approximately 85% of normal CBF). Angiogenesis was present in sites either immediately adjacent to or surrounded by the grafted cells. Our data indicate that map-ISODATA accurately and dynamically characterizes the ischemic lesion and its response to cell therapy.