The Drosophila TRPA channel, Painless, regulates sexual receptivity in virgin females.
The Drosophila TRPA channel, Painless, regulates sexual receptivity in virgin females.
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DOI：
10.1111/j.1601-183x.2009.00503.x
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发表时间：
2009-07
期刊：
影响因子：
--
通讯作者：
Aigaki T
中科院分区：
文献类型：
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作者：
Sakai T;Kasuya J;Kitamoto T;Aigaki T
瞬时受体电位(Trp)通道在感觉知觉中起着至关重要的作用。果蝇无痛(Pain)基因是哺乳动物TRPA1/ANKTM1基因的同源基因，在周围神经系统中的表达是避免有害高温或芥末行为所必需的。在这里，我们报告了在神经系统中表达的疼痛Trp通道在果蝇处女果蝇的性感受中的一个新的作用。与野生型雌蜂相比，疼痛突变雌蜂与野生型雄蜂交配的时间明显提前。野生型雄性与野生型和疼痛突变雌性相比，表现出相似的求偶潜伏期和求偶指数。因此，在野生型雄性和疼痛突变雌性中观察到的早期交配是疼痛突变雌性的性接受性增强的结果。通过在疼痛突变背景中表达疼痛转基因使雌性性接受能力恢复到野生型水平的救援实验，证实了疼痛参与了增强的雌性性接受能力。疼痛RNAi在推测的胆碱能或GABA能神经元中的靶向表达可以发现疼痛女性的突变表型。另一方面，疼痛RNAi在多巴胺能神经元中的靶向表达并不影响女性的性接受能力。此外，条件性抑制GABA能神经元的神经传递也会导致类似的性感受性增强。我们的结果表明，在胆碱能和/或GABA能神经元中表达的疼痛TRP通道与女性的性感受有关。
Transient receptor potential (TRP) channels play crucial roles in sensory perception. Expression of the Drosophila painless (pain) gene, a homolog of the mammalian TRPA1/ANKTM1 gene, in the peripheral nervous system is required for avoidance behavior of noxious heat or wasabi. Here we report a novel role of the Pain TRP channel expressed in the nervous system in the sexual receptivity in Drosophila virgin females. Compared with wild-type females, pain mutant females copulated with wild-type males significantly earlier. Wild-type males showed comparable courtship latency and courtship index toward wild-type and pain mutant females. Therefore, the early copulation observed in wild-type male and pain mutant female pairs is the result of enhanced sexual receptivity in pain mutant females. Involvement of pain in enhanced female sexual receptivity was confirmed by rescue experiments in which expression of a pain transgene in a pain mutant background restored the female sexual receptivity to the wild-type level. Targeted expression of pain RNAi in putative cholinergic or GABAergic neurons phenocopied the mutant phenotype of pain females. On the other hand, target expression of pain RNAi in dopaminergic neurons did not affect female sexual receptivity. In addition, conditional suppression of neurotransmission in putative GABAergic neurons resulted in a similar enhanced sexual receptivity. Our results suggest that Pain TRP channels expressed in cholinergic and/or GABAergic neurons are involved in female sexual receptivity.