An alkali-burn injury model of corneal neovascularization in the mouse.
An alkali-burn injury model of corneal neovascularization in the mouse.
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DOI：
10.3791/51159
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发表时间：
2014-04-07
期刊：
影响因子：
--
通讯作者：
Wang S
中科院分区：
文献类型：
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作者：
Anderson C;Zhou Q;Wang S
角膜新生血管（NV）可使多种视觉病变复杂化。利用可控的碱烧伤模型，可以产生可量化的角膜NV水平，用于角膜NV的机制研究和评估新血管疾病的潜在治疗方法。在正常情况下，角膜是无血管的，这种透明对于保持良好的视力是必不可少的。角膜新生血管形成（NV）可由外伤、角膜移植或传染病引起，破坏了所谓的角膜“血管生成特权”，形成了多种视觉病理的基础，甚至可能导致失明。虽然有几种治疗方法可供选择，但角膜新生血管病变的基本医疗需求仍未得到满足。为了开发安全、有效和有针对性的治疗方法，需要一个可靠的角膜NV模型和药物干预。在此，我们描述了一种碱烧伤小鼠角膜新生血管模型。本方案提供了一种方法，用于角膜控制碱烧伤，给药感兴趣的药理学化合物，并将结果可视化。该方法可为研究角膜NV和其他新生血管疾病的干预机制和机会提供工具。
Neovascularization (NV) of the cornea can complicate multiple visual pathologies. Utilizing a controlled, alkali-burn injury model, a quantifiable level of corneal NV can be produced for mechanistic study of corneal NV and evaluation of potential therapies for neovascular disorders. Under normal conditions, the cornea is avascular, and this transparency is essential for maintaining good visual acuity. Neovascularization (NV) of the cornea, which can be caused by trauma, keratoplasty or infectious disease, breaks down the so called ‘angiogenic privilege’ of the cornea and forms the basis of multiple visual pathologies that may even lead to blindness. Although there are several treatment options available, the fundamental medical need presented by corneal neovascular pathologies remains unmet. In order to develop safe, effective, and targeted therapies, a reliable model of corneal NV and pharmacological intervention is required. Here, we describe an alkali-burn injury corneal neovascularization model in the mouse. This protocol provides a method for the application of a controlled alkali-burn injury to the cornea, administration of a pharmacological compound of interest, and visualization of the result. This method could prove instrumental for studying the mechanisms and opportunities for intervention in corneal NV and other neovascular disorders.