Contribution of platelet-derived growth factor signaling to retina regeneration in zebrafish
Contribution of platelet-derived growth factor signaling to retina regeneration in zebrafish
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DOI：
10.1016/j.neulet.2020.134930
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发表时间：
2020-04
影响因子：
2.5
通讯作者：
Yuichi Saito;A. Yamaguchi;Shinsuke Nakamura;Hiroyuki Okuyoshi;M. Shimazawa;H. Hara
中科院分区：
文献类型：
--
作者：
Yuichi Saito;A. Yamaguchi;Shinsuke Nakamura;Hiroyuki Okuyoshi;M. Shimazawa;H. Hara
越来越多的证据表明，血小板衍生生长因子（PDGF）有助于各种类型的组织再生。然而，PDGF信号在视网膜再生中的作用和机制尚未得到充分的研究。为了澄清这一点，我们研究了PDGF信号在斑马鱼针刺后视网膜再生过程中的作用。时间过程分析显示，pdgf- a1在伤后6 h出现尖峰，pdgf- b1在伤后6 - 96 h出现宽峰。用AG1295抑制PDGF信号传导可在损伤后4天抑制brdu阳性增殖细胞数量。同时，通过AG1295处理，视网膜再生相关转录因子ascl1aandpax6b下调。玻璃体内注射重组PDGF-AA或-BB诱导斑马鱼细胞增殖。PDGF-BB注射诱导<s:1> ller胶质细胞源性神经源性簇；PDGF-AA增加了4c4阳性小胶质细胞。这些发现表明，PDGF信号传导有助于斑马鱼的视网膜再生，并导致不同类型的细胞增殖，这取决于PDGF的每种亚型。(160字)
Accumulated evidence indicates that platelet-derived growth factor (PDGF) contributes to various types of tissue regeneration. However, the effects and mechanisms of PDGF signaling for retina regeneration have not been sufficiently investigated. To clarify this, we investigated the role of PDGF signaling in retina regeneration process after needle puncture in zebrafish. Time-course analysis showed a spike peak ofpdgf-aat 6 h after injury and a broad peak ofpdgf-bduring 6–96 h after injury. Inhibition of PDGF signaling with AG1295 suppressed BrdU-positive proliferative cell numbers at 4 days after injury. At the same time, retina regeneration-associated transcription factors,ascl1aandpax6b, were down-regulated by AG1295 treatment. Intravitreal injection of human recombinant PDGF-AA or -BB into intact zebrafish induced the cell proliferation. PDGF-BB injection induced the Müller glia-derived neurogenic cluster; PDGF-AA increased the 4C4-positive microglia. These findings indicate that PDGF signaling contributes to retina regeneration in zebrafish and causes different types of cell proliferation, depending on each subtype of PDGF. (160 words)