The dependence of radiation enhancement effect on the concentration of gold nanoparticles exposed to low- and high-LET radiations
The dependence of radiation enhancement effect on the concentration of gold nanoparticles exposed to low- and high-LET radiations
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辐射增强效应对暴露于低和高 LET 辐射的金纳米颗粒浓度的依赖性
DOI：
10.1016/j.ejmp.2015.01.006
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发表时间：
2015-05-01
影响因子：
3.4
通讯作者：
Li, Qiang
中科院分区：
文献类型：
--
作者：
Liu, Yan;Liu, Xi;Li, Qiang
我们研究了羟基自由基的产生对15 nm柠檬酸盐封端的金纳米粒子的浓度和剂量的依赖性，使用香豆素-3-羧酸在磷酸盐缓冲盐水（PBS）中，并通过X射线和碳离子的克隆形成存活测定研究了不同浓度的金纳米粒子对人宫颈癌HeLa细胞的放射增敏作用。金纳米粒子的羟基自由基的生产的增强因子达到最大值3.66的X-射线在浓度为0.1毫克/毫升，而最大值为5.52的碳离子在1.0微克/毫升金纳米粒子在PBS中的存在下。当细胞与1.5-15.0 μ g/mL金纳米颗粒共培养时，在50%存活水平下，X射线和碳离子的敏化增强比分别为1.14 - 2.88和1.27 - 1.44。我们的数据表明，金纳米粒子显示在低和高LET辐射的羟基自由基的产生和细胞杀伤方面的放射增敏作用。金纳米粒子在PBS和细胞中的浓度在放射增敏效应中起重要作用。基于在PBS中的AuNPs可以增加羟自由基的产生，并且没有观察到细胞在G（2）/M期的积累，我们推断AuNPs增加羟自由基的产生提供了放射增敏的机制。(C)2015年意大利医学协会。由爱思唯尔有限公司出版。保留所有权利。
We examined the dependence of hydroxyl radical production on the concentration of 15 nm citrate-capped AuNPs and dose using coumarin-3-carboxylic acid in phosphate buffered saline (PBS), and investigated the radiosensitisation of different concentration AuNPs on human cervix carcinoma HeLa cells through clonogenic survival assay for X-rays and carbon ions. The enhancement factor of AuNPs for hydroxyl radical production reached a maximum 3.66 for X-rays at the concentration of 0.1 mg/mL while the maximum was 5.52 for carbon ions in presence of 1.0 mu g/mL AuNPs in PBS. At 50% survival level, the sensitizer enhancement ratios of X-rays and carbon ions varied from 1.14 to 2.88 and from 1.27 to 1.44, respectively, when cells were co-cultured with 1.5-15.0 mu g/mL AuNPs. Our data indicate AuNPs showed radiosensitisation in terms of hydroxyl radical production and cell killing for low- and high-LET radiations. The concentration of AuNPs in PBS and cells played an important role in radiosensitizing effect. Based on the fact-the AuNPs in PBS could improve the production of hydroxyl radical and no accumulation of cells in the G(2)/M phase was observed, we deduce that the increment of hydroxyl radical production with AuNPs provided a mechanism for radiosensitisation. (C) 2015 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.