ISOLATION AND COMPARISON OF BOVINE PARVOVIRUSES
ISOLATION AND COMPARISON OF BOVINE PARVOVIRUSES
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DOI：
10.1093/infdis/126.5.531
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发表时间：
1972-01-01
影响因子：
6.4
通讯作者：
REED, DE
中科院分区：
文献类型：
--
作者：
BATES, RC;STORZ, J;REED, DE
自1959年分离出牛细小病毒1号以来，还没有从牛身上分离出细小病毒的报道，尽管血清学调查表明细小病毒经常感染牛。将患有肠道疾病小牛的粪便和肠道样本接种到牛胎肺（BFL）细胞、牛胎肾（BFK）细胞和猴肾细胞系的培养物上。分离出病毒33例。其中10个分离株被鉴定为牛细小病毒，其余分离株暂定为牛肠病毒。BFL细胞对牛细小病毒的分离敏感性高于BFK细胞。从混合物中分离出两种牛细小病毒。在有肠道病毒抗血清的情况下，再培养样品感染肠道病毒。细小病毒分离物在豚鼠和人O型血细胞的血凝和血吸附、codry型A的形成、feulgen阳性核内包涵体（用异硫氰酸荧光素标记的牛细小病毒1抗体染色）、牛细小病毒抗血清对HA和传染性的影响以及5-溴-2 ' -脱氧尿苷对复制的抑制作用对其进行了鉴定。
Isolations of parvovirus from cattle have not been reported since the isolation of bovine parvovirus 1 in 1959, although serologic surveys indicated that parvoviruses frequently infect cattle. Fecal and intestinal samples from calves with enteric diseases were inoculated onto bovine fetal lung (BFL) cells, bovine fetal kidney (BFK) cells, and cultures of a monkey kidney-cell line. Virus was isolated in 33 cases. Ten of these isolates were identified as bovine parvoviruses, and the remaining isolates were tentatively classified as bovine enteroviruses. BFL cells were more sensitive than BFK cells for the isolation of bovine parvoviruses. Two bovine parvoviruses were recovered from mixed .infections with enterovirus by reculture of the samples in the presence of antiserum to enterovirus. The parvovirus isolates were characterized on the basis of hemagglutination and hemadsorption of guinea-pig and human type O blood cells, development of Cowdry type A, Feulgen-positive intranuclear inclusions (which stained with fluorescein-isothiocyanate-labeled antibodies to bovine parvovirus 1), the effect of bovine antiserum to parvovirus on HA and infectivity, and the inhibition of replication by 5-bromo-2′-deoxyuridine.