Microbially-enhanced chemisorption of heavy metals : A method for the bioremediation of solutions containing long-lived isotopes of neptunium and plutonium
Microbially-enhanced chemisorption of heavy metals : A method for the bioremediation of solutions containing long-lived isotopes of neptunium and plutonium
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DOI：
10.1021/es9708528
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发表时间：
1998
影响因子：
11.4
通讯作者：
L. Macaskie;G. Bašnáková
中科院分区：
文献类型：
--
作者：
L. Macaskie;G. Bašnáková
柠檬酸杆菌属的固定化细胞使用已建立的技术从溶液中去除可忽略的镎和钚，该技术使用生物产生的磷酸盐配体（Pi）进行金属磷酸盐生物沉淀。这些超铀放射性核素的去除增强了事先暴露的生物质镧在有机磷酸盐底物的存在下，形成细胞结合的磷酸镧。聚丙烯酰胺凝胶固定化细胞本身去除很少的Np和Pu，但预载的LaPO4促进去除Np和Pu后，随后的挑战，在流通柱。当在10 mL后停止实验时，每1 mL柱加载约2 μg Np，保持约90%的输入金属去除。通过这种技术去除超铀元素，一般被描述为微生物增强重金属化学吸附（MECHM），是通过生物累积和化学吸附机制的混合。
Immobilized cells of a Citrobacter sp. removed neptunium and plutonium negligibly from solution using an established technique that used biologically-produced phosphate ligand (Pi) for metal phosphate bioprecipitation. Removal of these transuranic radionuclides was enhanced by prior exposure of the biomass to lanthanum in the presence of organophosphate substrate to form cell-bound LaPO4. Polyacrylamide gel-immobilized cells removed little Np and Pu per se, but preloaded LaPO4 promoted the removal of Np and Pu upon subsequent challenge in a flow-through column. Approximately 2 μg of Np was loaded per 1 mL, column, when the experiments were stopped after 10 mL, with maintenance of approximately 90% removal of the input metal. Transuranic element removal by this technique, generically described as microbially-enhanced chemisorption of heavy metals (MECHM), is via a hybrid of bioaccumulative and chemisorptive mechanisms.