Histochemical and immunohistochemical analysis of the mechanism of calcification of Meckel's cartilage during mandible development in rodents
Histochemical and immunohistochemical analysis of the mechanism of calcification of Meckel's cartilage during mandible development in rodents
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DOI：
10.1046/j.1469-7580.1999.19420265.x
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发表时间：
1999-02-01
影响因子：
2.4
通讯作者：
Nawa, T
中科院分区：
文献类型：
--
作者：
Ishizeki, K;Saito, H;Nawa, T
人们普遍认为，哺乳动物的梅克尔软骨是未钙化的透明软骨，会被吸收并且不参与下颌骨的骨形成。我们使用组织化学和免疫细胞化学方法、电子显微镜和电子探针微量分析仪检查了梅克尔软骨基质钙化的空间和时间特征。相对于颏孔下方骨化起始部位，梅克尔软骨的下颌内部分可以示意性地分为前部和后部。基质钙化发生在可以通过光学和电子显微镜以及免疫组织化学染色检测到碱性磷酸酶活性的区域。 X型胶原的表达仅限于下颌内Meckel软骨的肥大细胞，茜素红和von Kossa染色显示钙化从原发骨化中心向前和向后两个方向进展。钙化软骨基质被细胞主动吸收后，从软骨膜骨领侵入的骨小梁形成新的骨岛。除了骨桥蛋白的免疫染色结果之外，针对 I 型和 II 型胶原蛋白的双重免疫荧光染色结果也支持了这种骨形成的证据。后部的钙化与下颌内梅克尔软骨前部的钙化相似，我们的研究结果表明后部也通过软骨内型钙化机制促进下颌骨的骨形成。
It is widely accepted that Meckel's cartilage in mammals is uncalcified hyaline cartilage that is resorbed and is not involved in bone formation of the mandible. We examined the spatial and temporal characteristics of matrix calcification in Meckel's cartilage, using histochemical and immunocytochemical methods, electron microscopy and an electron probe microanalyser. The intramandibular portion of Meckel's cartilage could be divided schematically into anterior and posterior portions with respect to the site of initiation of ossification beneath the mental foramen. Calcification of the matrix occurred in areas in which alkaline phosphatase activity could be detected by light and electron microscopy and by immunohistochemical staining. The expression of type X collagen was restricted to the hypertrophic cells of intramandibular Meckel's cartilage, and staining with alizarin red and von Kossa stain revealed that calcification progressed in both posterior and anterior directions from the primary centre of ossification. After the active cellular resorption of calcified cartilage matrix, new osseous islands were formed by trabecular bone that intruded from the perichondrial bone collar. Evidence of such formation of bone was supported by results of double immunofluorescence staining specific for type I and type II collagens, in addition to results of immunostaining for osteopontin. Calcification of the posterior portion resembled that in the anterior portion of intramandibular Meckel's cartilage, and our findings indicate that the posterior portion also contributes to the bone formation of the mandible by an endochondral-type mechanism of calcification.