IMPACT OF REACTIVE OXYGEN SPECIES ON SPERMATOZOA - A BALANCING ACT BETWEEN BENEFICIAL AND DETRIMENTAL EFFECTS
IMPACT OF REACTIVE OXYGEN SPECIES ON SPERMATOZOA - A BALANCING ACT BETWEEN BENEFICIAL AND DETRIMENTAL EFFECTS
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DOI：
10.1093/humrep/10.suppl_1.15
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发表时间：
1995-10-01
影响因子：
6.1
通讯作者：
GAGNON, C
中科院分区：
文献类型：
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作者：
DELAMIRANDE, E;GAGNON, C
活性氧（ROS）对精子功能有有益或有害的影响，这取决于所涉及的ROS的性质和浓度，以及接触的时刻和位置。精液中过量产生ROS，主要是由中性粒细胞产生的，也可以由异常精子产生，可能是导致不育的原因。过氧化氢是人类精子的主要有毒ROS。低浓度的ROS不影响精子的生存能力，但主要通过细胞内ATP的消耗和随后轴突蛋白磷酸化的降低导致精子固定化。高浓度的过氧化氢诱导脂质过氧化并导致细胞死亡。另一方面，超氧阴离子似乎在超活化和能化的发展中起主要作用，观察到：(1)外源产生的超氧阴离子诱导超活化和能化；（ii）使能精子本身在长时间内产生高浓度的超氧阴离子；（iii）通过超氧化物歧化酶去除这种活性氧可防止各种生物体液引起的精子过度活化和获能，强调超氧化物阴离子在这些过程中的重要性。
Reactive oxygen species (ROS) have beneficial or detrimental effects on sperm functions depending on the nature and the concentration of the ROS involved, as well as the moment and the location of exposure, Excessive generation of ROS in semen, mainly by neutrophils but also by abnormal spermatozoa, could be a cause for infertility, Hydrogen peroxide is the primary toxic ROS for human spermatozoa. Low concentrations of this ROS do not affect sperm viability but cause sperm immobilization mostly via depletion of intracellular ATP and the subsequent decrease in the phosphorylation of axonemal proteins. High concentrations of hydrogen peroxide induce lipid peroxidation and result in cell death. On the other hand, the superoxide anion appears to play a major role in the development of hyperactivation and capacitation, The observations that: (i) exogenously generated superoxide anions induce hyperactivation and capacitation; (ii) capacitating spermatozoa themselves produce elevated concentrations of superoxide anion over prolonged periods of time; and (iii) removal of this ROS by superoxide dismutase prevents sperm hyperactivation and capacitation induced by various biological fluids, stress the importance of the superoxide anion in these processes.