Toxicity and bioavailability of copper nanoparticles to the terrestrial plants mung bean (Phaseolus radiatus) and wheat (Triticum aestivum): Plant agar test for water-insoluble nanoparticles
Toxicity and bioavailability of copper nanoparticles to the terrestrial plants mung bean (Phaseolus radiatus) and wheat (Triticum aestivum): Plant agar test for water-insoluble nanoparticles
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DOI：
10.1897/07-481.1
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发表时间：
2008-09-01
影响因子：
4.1
通讯作者：
Kweon, Hee-Seok
中科院分区：
文献类型：
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作者：
Lee, Woo-Mi;An, Youn-Joo;Kweon, Hee-Seok
由于纳米颗粒不溶于水，其毒性实验受到限制。本研究展示了植物琼脂测试，用于纳米粒子均匀暴露于植物物种。研究了纳米铜对植物幼苗生长的影响，并研究了纳米颗粒的生物累积性。所有测试均在植物琼脂培养基中进行，以防止测试单元中水不溶性纳米粒子沉淀。植物种类为绿豆（绿豆）和小麦（小麦）。研究了暴露于不同浓度的铜纳米颗粒对幼苗生长的抑制作用。铜纳米粒子对这两种植物都有毒，并且具有生物可利用性。暴露于 Cu 纳米粒子的 P. radiatus 和 T. aestivum 的 2 d 中位有效浓度分别为 335（95% 置信水平，251 - 447）和 570（450 - 722）mg/L。绿豆对铜纳米粒子比普通小麦更敏感。从 Cu 纳米颗粒释放的铜离子在本研究的浓度范围内的影响可以忽略不计，并且明显的毒性明显是由 Cu 纳米颗粒引起的。随着铜纳米颗粒浓度的增加，生物累积性增加，并且使用透射电子显微镜-能量色散光谱在细胞中观察到颗粒的聚集。本研究表明，植物琼脂测试是测试难溶于水的纳米粒子植物毒性的良好方案。
Because of their insolubility in water, nanoparticles have a limitation concerning toxicity experiments. The present study demonstrated a plant agar test for homogeneous exposure of nanoparticles to plant species. The effect of Cu nanoparticles on the growth of a plant seedling was studied, and bioaccumulation of nanoparticles was investigated. All tests were conducted in plant agar media to prevent precipitation of water-insoluble nanoparticles in test units. The plant species were Phaseolus radiatus (mung bean) and Triticum aestivum ( wheat). Growth inhibition of a seedling exposed to different concentrations of Cu nanoparticles was examined. Copper nanoparticles were toxic to both plants and also were bioavailable. The 2-d median effective concentrations for P. radiatus and T. aestivum exposed to Cu nanoparticles were 335 (95% confidence level, 251 - 447) and 570 ( 450 - 722) mg/L, respectively. Phaseolus radiatus was more sensitive than T. aestivum to Cu nanoparticles. A cupric ion released from Cu nanoparticles had negligible effects in the concentration ranges of the present study, and the apparent toxicity clearly resulted from Cu nanoparticles. Bioaccumulation increased with increasing concentration of Cu nanoparticles, and agglomeration of particles was observed in the cells using transmission-electron microscopy-energy-dispersive spectroscopy. The present study demonstrated that the plant agar test was a good protocol for testing the phytotoxicity of nanoparticles, which are hardly water soluble.