Enhanced microfiltration devices configured with hydrodynamic trapping and a rain drop bypass filtering architecture for microbial cells detection
Enhanced microfiltration devices configured with hydrodynamic trapping and a rain drop bypass filtering architecture for microbial cells detection
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DOI：
10.1039/b800015h
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Liu, Wen-Tso
中科院分区：
文献类型：
--
作者：
Lay, Christophe;Teo, Cheng Yong;Liu, Wen-Tso
需要超细（< 1 μ m）微过滤器来有效地捕获微生物细胞。我们设计了具有雨滴旁路结构的微滤波器，以有限的电池损失为代价，显着降低了堵塞的可能性。与我们上一代的微滤设备相比，新的雨滴旁路结构配置具有显着降低的压降，并且可以更好地捕获原生动物细胞（小隐孢子虫和贾第鞭毛虫）。一种显示亚微米过滤器间隙的改进版本被用于捕获和检测细菌细胞（大肠杆菌），通过细胞标记方法，旨在放大荧光信号发射，从而提高检测灵敏度。
Ultra-fine (< 1 mu m) microfilters are required to effectively trap microbial cells. We designed microfilters featuring a rain drop bypass architecture, which significantly reduces the likelihood of clogging at the cost of limited cell loss. The new rain drop bypass architecture configuration has a substantially lower pressure drop and allows a better efficiency in trapping protozoan cells ( Cryptosporidium parvum and Giardia lamblia) in comparison to our previous generation of a microfilter device. A modified version displaying sub-micron filter gaps was adapted to trap and detect bacterial cells ( Escherichia coli), through a method of cells labeling, which aims to amplify the fluorescence signal emission and therefore the sensitivity of detection.