Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry
Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry
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DOI：
10.1016/j.jchromb.2008.05.034
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发表时间：
2008-07-01
影响因子：
3
通讯作者：
Goto, Junichi
中科院分区：
文献类型：
--
作者：
Hishinuma, Takanori;Suzuki, Kaori;Goto, Junichi
开发了一种快速、简单、高灵敏度的方法，采用高效液相色谱/串联质谱（LC/MS/MS）定量测定20 μ L人血清中兰索拉唑和雷贝拉唑的浓度。在C18分析柱上，使用乙腈/1 mM甲酸铵（140/60，v/v）移动的相分离分析物沿着内标物（兰索拉唑氘衍生物），并在选定的反应监测（SRM）模式下进行分析。定量下限为0.25 ng/mL。对于每种分析物（从0.25 ng到2.5 μ g/mL），观察到良好的线性响应。该方法可用于治疗药物监测和药代动力学研究。(C)2008 Elsevier B. V.保留所有权利。
A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20 mu L of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1 mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25 ng/mL. A good linear response was observed for each analyte (from 0.25 ng to 2.5 mu g/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies. (C) 2008 Elsevier B.V. All rights reserved.