The antigenic determinant of the LH-releasing hormone for three different antisera.
The antigenic determinant of the LH-releasing hormone for three different antisera.
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三种不同抗血清的 LH 释放激素的抗原决定簇。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
N. Sukura
中科院分区：
文献类型：
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作者：
A. Arimura;H. Sato;D. Coy;R. Worobec;A. Schally;N. Yanaihara;T. Hashimoto;C. Yanaihara;N. Sukura
通过使用针对 LH-RH 十肽的 3 种不同抗血清在 LH-RH 放射免疫测定 (RIA) 系统中测试 LH-RH 类似物和片段的交叉反应，研究了 LH 释放激素 (LH-RH) 的抗原决定簇。兔抗血清 No. 419 是针对吸附在聚乙烯吡咯烷酮 (PVP) 上的 LH-RH 产生的。抗血清编号 710 和 742 是通过用 LH-RH 免疫兔子而产生的，LH-RH 分别通过其 C 末端与牛血清白蛋白缀合，或通过 N 末端与人血清白蛋白缀合。对于419号抗血清，发现LH-RH的1位和2位上的N-末端(焦)-谷氨酸和/或组氨酸分别增强抗原-抗体相互作用，但并不是必不可少的。同样，C 端酰胺和甘氨酸-NH2 在这些相互作用中并未发挥主要作用。 LH-RH 七肽片段（对应于位置 3 至 9 的氨基酸序列）在该 RIA 系统中显示出与 LH-RH 的交叉反应性，尽管需要比冷 LH-RH 更大的量才能对标记的 LH-RH 的结合进行可比较的抑制。对于710号抗血清，对应于位置2至7的LH-RH六肽片段显示出相当大的交叉反应性。 2 位的组氨酸发挥了重要作用，但 C 末端的酰胺基团和甘氨酸酰胺基团都不是必需的。对于742号抗血清，LH-RH的C端四肽酰胺片段在LH-RH中表现出相当大的交叉反应性，酰胺部分本身至关重要。这些抗血清可用于研究生物材料中与 LH-RH 相关的肽。
Antigenic determinants of LH-releasing hormone (LH-RH) were investigated by testing the cross-reaction of LH-RH analogues and fragments in LH-RH radioimmunoassay (RIA) systems using 3 different antisera against the LH-RH decapeptide. Rabbit antiserum No. 419 was generated against LH-RH adsorbed on polyvinylpyrrolidone (PVP). Antisera Nos. 710 and 742 were produced by immunizing rabbits with LH-RH conjugated either with bovine serum albumin through its C-terminus, or with human serum albumin through the N-terminus, respectively. For antiserum No. 419, the N-terminal (pyro)-glutamic acid and/or histidine in positions 1 and 2 of LH-RH, respectively, were found to enhance the antigen-antibody interaction, but were not indispensable for it. Similarly, the C-terminal amide and glycine-NH2 did not play a major role in these interactions. The LH-RH heptapeptide fragment, corresponding to amino acid sequence from positions 3 to 9, showed a cross-reactivity in this RIA system with LH-RH, although greater amounts than those of cold LH-RH were required for a comparable inhibition of binding of labelled LH-RH. For antiserum No. 710, the LH-RH hexapeptide fragment corresponding to positions 2 to 7 showed considerable cross-reactivity. Histidine in position 2 played an important role but neither the amide group nor the glycine amide group at the C-terminus were essential. For antiserum No. 742, the C-terminal tetrapeptide-amide fragment of LH-RH showed considerable cross-reactivity in the LH-RH, the amide moiety itself being of crucial importance. These antisera may be useful in investigating peptides related to LH-RH in biological materials.