[Study on the purification and scavenging free radical activity of water soluble polysaccharide from Ligularia hodgsonii].
[Study on the purification and scavenging free radical activity of water soluble polysaccharide from Ligularia hodgsonii].
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发表时间：
2010-09
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通讯作者：
Chun-lan Liu;Yang Li;N. Du;Xiao Huang;Wen-Shu Wang
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作者：
Chun-lan Liu;Yang Li;N. Du;Xiao Huang;Wen-Shu Wang
目的为进一步开发利用霍氏药材提供参考。方法采用热水提取、乙醇提取的方法从厚朴中提取粗多糖LW。对粗多糖进行了分离纯化，得到LW21。用SepharoseCL-4B柱层析、纸层析和醋酸纤维凝胶电泳法证实了其均一性。用荧光法测定了多糖对*OH和O2-*的清除作用。结果抗氧化实验表明，LW和LW21具有清除超氧阴离子自由基和羟基自由基的作用。LW21对超氧阴离子自由基和羟基自由基的清除能力高于LW。结论和黄连多糖具有较强的抗氧化活性，其抗氧化活性具有一定的量效关系。
OBJECTIVE The study was to provided the reference for further development and utilization of Ligularia hodgsonii. METHODS The crude polysaccharide LW was extracted from Lugularia hodgsonii with hot water, and prectated by ethanol. The crude polysaccharide has been fractionted and purified, so LW21 was got. Its homogeneity was confirmed by chromatography on Sepharose CL-4B, paper chromatography and cellulous actate electrophoresis. The scavenging effets of the polysacehardes on * OH and O2-* were determined by fluorescence method. RESULTS Antioxidative experiment shows that LW and LW21 were effective in scavenging superoxide radical and hydroxyl radical. The scavenging capacity for superoxide radical and hydroxyl radical of LW21 had higher than that of LW. CONCLUSION The polysaceharide from Ligularia hodgsonii had stronger antioxidant actioxdant activities, and their antioxidant activities had certain dose-relationship.