IDENTIFICATION OF A NOVEL CELLULAR COFACTOR FOR THE REV/REX CLASS OF RETROVIRAL REGULATORY PROTEINS
IDENTIFICATION OF A NOVEL CELLULAR COFACTOR FOR THE REV/REX CLASS OF RETROVIRAL REGULATORY PROTEINS
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DOI：
10.1016/0092-8674(95)90437-9
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发表时间：
1995-08-11
期刊：
影响因子：
64.5
通讯作者：
CULLEN, BR
中科院分区：
文献类型：
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作者：
BOGERD, HP;FRIDELL, RA;CULLEN, BR
HIV-1 Rev是一类逆转录病毒调节蛋白的原型，其诱导靶RNA的序列特异性核输出。该功能需要Rev激活结构域，据信其结合必需的细胞辅因子。我们报告了一种新的人类基因产物的鉴定，该产物不仅在体外和体内与HIV-1 Rev激活结构域结合，而且与其他Rev和雷克斯蛋白中的功能等同结构域结合。Rev/雷克斯激活结构域结合（Rab）蛋白占据HIV-I Rev上的结合位点，该结合位点与遗传分析预测的结合位点精确匹配。当Rev组装到其RNA靶标上时，Rab结合Rev激活结构域，并且当过表达时可以显著增强Rev活性。我们的结论是，Rab是预测的激活域特异性辅因子的Rev/雷克斯类RNA输出因子。
HIV-1 Rev is the prototype of a class of retroviral regulatory proteins that induce the sequence-specific nuclear export of target RNAs. This function requires the Rev activation domain, which is believed to bind an essential cellular cofactor. We report the identification of a novel human gene product that binds to not only the HIV-1 Rev activation domain in vitro and in vivo but also to functionally equivalent domains in other Rev and Rex proteins. The Rev/Rex activation domain-binding (Rab) protein occupies a binding site on HIV-I Rev that precisely matches that predicted by genetic analysis. Rab binds the Rev activation domain when Rev is assembled onto its RNA target and can significantly enhance Rev activity when overexpressed. We conclude that Rab is the predicted activation domain-specific cofactor for the Rev/Rex class of RNA export factors.