Interstitial glucose concentration and glycemia: implications for continuous subcutaneous glucose monitoring
Interstitial glucose concentration and glycemia: implications for continuous subcutaneous glucose monitoring
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DOI：
10.1152/ajpendo.2000.278.4.e716
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发表时间：
2000-04-01
影响因子：
5.1
通讯作者：
Reach, G
中科院分区：
文献类型：
--
作者：
Aussedat, B;Dupire-Angel, M;Reach, G
在麻醉的非糖尿病大鼠中研究了用小型皮下葡萄糖传感器测定的血浆葡萄糖浓度和间质葡萄糖浓度的变化。通过两种不同的校准程序估计间质葡萄糖。首先，葡萄糖负荷后，通过单点校准程序估计，间质葡萄糖增加的幅度是血浆葡萄糖增加的 70%。我们认为这是由于内源性胰岛素对外周葡萄糖摄取的影响所致。其次，在葡萄糖负荷后血浆葡萄糖自发继发性下降过程中，间质葡萄糖下降速度快于血浆葡萄糖，这也可能是由于胰岛素对外周葡萄糖摄取的影响所致。第三，在胰岛素引起的低血糖期间，间质葡萄糖的下降不如血浆葡萄糖明显，这表明低血糖抑制了葡萄糖向间质组织的转移。随后，间质葡萄糖在恢复到基础值期间仍然低于血浆葡萄糖，这表明胰岛素对外周葡萄糖摄取的刺激作用是持久的。如果在大鼠中获得的这些观察结果与人类生理学相关，那么血浆和间质葡萄糖浓度之间的这种差异可能对皮下葡萄糖传感器在糖尿病患者连续血糖监测中的使用产生重大影响。
The changes in plasma glucose concentration and in interstitial glucose concentration, determined with a miniaturized subcutaneous glucose sensor, were investigated in anesthetized nondiabetic rats. Interstitial glucose was estimated through two different calibration procedures. First, after a glucose load, the magnitude of the increase in interstitial glucose, estimated through a one-point calibration procedure, was 70% of that in plasma glucose. We propose that this is due to the effect of endogenous insulin on peripheral glucose uptake. Second, during the spontaneous secondary decrease in plasma glucose after the glucose load, interstitial glucose decreased faster than plasma glucose, which may also be due to the effect of insulin on peripheral glucose uptake. Third, during insulin-induced hypoglycemia, the decrease in interstitial glucose was less marked than that of plasma glucose, suggesting that hypoglycemia suppressed transfer of glucose into the interstitial tissue; subsequently, interstitial glucose remained lower than plasma glucose during its return to basal value, suggesting that the stimulatory effect of insulin on peripheral glucose uptake was protracted. If these observations obtained in rats are relevant to human physiology, such discrepancies between plasma and interstitial glucose concentration may have major implications for the use of a subcutaneous glucose sensor in continuous blood glucose monitoring in diabetic patients.