Potassium channel blocker inhibits the formation and electroactivity of Geobacter biofilm
Potassium channel blocker inhibits the formation and electroactivity of Geobacter biofilm
复制标题
钾通道阻滞剂抑制地杆菌生物膜的形成和电活性
DOI：
10.1016/j.scitotenv.2019.135796
复制
发表时间：
2020-02-25
影响因子：
9.8
通讯作者：
Zhou, Shungui
中科院分区：
文献类型：
--
作者：
Jing, Xianyue;Yang, Yuting;Zhou, Shungui
生物膜中的细菌能够利用钾离子通道介导的电信号来实现细胞间的通讯。然而，目前还不清楚这些信号是否在被强电场包围时在Geophyssp中发挥作用。本研究使用钾通道阻断剂（四乙基铵，TEA），其干扰K'的释放但不干扰细菌生长，以证明钾离子通道介导的电信号传导影响Gesterol sulfurreducens的形成和电活性。结果表明，5 mM TEA减缓了Geophylla sulfreducens生物膜的形成，并且电流密度比对照低约50%。电化学分析表明，添加TEA的生物膜的电活性较差。特别地，具有TEA的微米级生物膜表现出更少的高电流峰，并且在Geculumsulfreducens生物膜中参与电子转移的最外层基团的种类与对照不同。本工作为揭示钾通道在还原硫锗酸盐电活性生物膜中的作用提供了初步证据。(C)2019爱思唯尔B.V.保留所有权利。
Bacteria in biofilms are able to utilize potassium ion channel-mediated electrical signaling to achieve cell-cell communication. However, it remains unclear whether these signals play a role in Geobacter sp. when surrounded by an intense electric field. This study used a potassium channel blocker (tetraethylammonium, TEA) that interfered with the release of K' but not bacterial growth to demonstrate that potassium ion channel-mediated electrical signaling affected the formation and electroactivity of Geobacter sulfurreducens. The results showed that 5 mM TEA slowed the formation of Geobacter sulfurreducens biofilm, and the current density was -50% lower than in the control. The electrochemical analyses showed that the electroactivity of the biofilms with TEA addition was inferior. In particular, the micrometer- scale biofilm with TEA exhibited fewer high current peaks, and the species of outermost groups that participated in the electron transfer in Geobacter sulfurreducens biofilms was different from the control. This work provides initial evidence to reveal the role of potassium channels in Geobacter sulfurreducens electroactive biofilms. (C) 2019 Elsevier B.V. All rights reserved.