Plasmodium knowlesi - Clinical Isolate Genome Sequencing to Inform Translational Same-Species Model System for Severe Malaria.
Plasmodium knowlesi - Clinical Isolate Genome Sequencing to Inform Translational Same-Species Model System for Severe Malaria.
复制标题
诺氏疟原虫——临床分离物基因组测序为重症疟疾同种模型系统的翻译提供信息。
DOI：
10.3389/fcimb.2021.607686
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发表时间：
2021
影响因子：
5.7
通讯作者：
Cox-Singh J
中科院分区：
文献类型：
--
作者：
Oresegun DR;Daneshvar C;Cox-Singh J
疟疾造成了令人无法接受的高发病率和死亡率，特别是在撒哈拉以南非洲国家。疟疾是由疟原虫属的成员物种引起的，尽管人们作出了协调一致的努力，有时甚至是勇敢的努力，但人们对导致严重疾病的潜在病理生理过程知之甚少。在这里，我们描述了由诺氏疟原虫引起的人畜共患疟疾和这种寄生虫作为严重疟疾模型系统的效用。我们提出了一种使用MinION平台从存档的临床样本中生成长读第三代疟原虫基因组序列数据的方法。这种方法和技术是可获得的、负担得起的，而且数据是实时生成的。我们建议通过广泛采用这种方法，从地理上不同的研究地点获得有关临床相关寄生虫多样性的重要信息，包括多个基因家族成员。随着时间的推移，我们的目标是利用诺氏疟原虫作为一种广泛使用的实验室模型和人类病原体的双重特性，开发一种具有代表性的严重疟疾转化模型系统，该系统由临床相关的寄生虫多样性提供信息。
Malaria is responsible for unacceptably high morbidity and mortality, especially in Sub-Saharan African Nations. Malaria is caused by member species’ of the genus Plasmodium and despite concerted and at times valiant efforts, the underlying pathophysiological processes leading to severe disease are poorly understood. Here we describe zoonotic malaria caused by Plasmodium knowlesi and the utility of this parasite as a model system for severe malaria. We present a method to generate long-read third-generation Plasmodium genome sequence data from archived clinical samples using the MinION platform. The method and technology are accessible, affordable and data is generated in real-time. We propose that by widely adopting this methodology important information on clinically relevant parasite diversity, including multiple gene family members, from geographically distinct study sites will emerge. Our goal, over time, is to exploit the duality of P. knowlesi as a well-used laboratory model and human pathogen to develop a representative translational model system for severe malaria that is informed by clinically relevant parasite diversity.