Three Metabolites from the Mangrove Endophytic Fungus Sporothrix sp (#4335) from the South China Sea
Three Metabolites from the Mangrove Endophytic Fungus Sporothrix sp (#4335) from the South China Sea
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DOI：
10.1021/jo802096q
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发表时间：
2009-02-06
影响因子：
3.6
通讯作者：
Lin, Yongcheng
中科院分区：
文献类型：
--
作者：
Wen, Lu;Cai, Xiaoling;Lin, Yongcheng
从红树林内生真菌孢子丝菌属（#4335）中分离出三种代谢产物孢子丝菌素A、B和C（1-3）。通过光谱数据和X射线衍射技术鉴定了它们的结构，其中化合物1显示出强烈的乙酰胆碱酯酶抑制作用。1，3，6，8-四羟基萘（T4 HN）被认为是1和2生物合成的前体之一。在初级生物合成基因研究中，用LC 1-Im/2c-Im引物对获得的部分基因片段显示与编码T4 HN合酶的基因密切相关。推导的蛋白质序列与其他参与T4 HN生物合成的真菌PKS基因的酮合酶结构域高度同源。
Three metabolites, sporothrins A, B, and C (1-3), were isolated from the mangrove endophytic fungus Sporothrix sp. (#4335). Their structures were identified by the spectral data and X-ray diffractive techniques, with compound 1 showing strong inhibition of acetylcholine esterase. 1,3,6,8-Tetrahydroxynaphthalene (T4HN) was deduced as one of the precursors in the biosynthesis of 1 and 2. In a primary biosynthesis gene study, the partial gene fragment obtained with the LC1-Im/2c-Im primer pair was shown to be closely related to genes encoding T4HN synthase. The deduced protein sequences were highly homologous to the ketosynthase domains of other fungal PKS genes involved in T4HN biosynthesis.