Traveling wave formation in vertebrate segmentation
Traveling wave formation in vertebrate segmentation
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DOI：
10.1016/j.jtbi.2009.01.003
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发表时间：
2009-04-07
影响因子：
2
通讯作者：
Iwasa, Yoh
中科院分区：
文献类型：
--
作者：
Uriu, Koichiro;Morishita, Yoshihiro;Iwasa, Yoh
在脊椎动物的体节发生中，“分段时钟”基因（如斑马鱼中的her，小鸡中的hairy和小鼠中的hes）显示振荡，通过细胞-细胞相互作用与附近的细胞同步。高表达基因的位置以规则的间隔出现，并像波浪一样从后向前移动，向前端速度减慢。当基因-蛋白质动力学中的一个反应速率存在前后梯度时，我们分析了她的基因表达的行波模式。我们采用了一个模型，其中包括mRNA和蛋白质在每个细胞中的动力学和细胞与细胞之间的相互作用，通过Delta-Notch系统明确。我们发现，所观察到的时空模式可以解释，如果mRNA降解，蛋白质翻译，蛋白质运输到细胞核发生更快，或mRNA转录，Delta蛋白质合成发生在后方比在前面的区域慢。所有这些梯度都是那些在前部比在后部产生更长的时钟基因表达振荡周期的梯度。基于这一结果，我们推导出了基因表达的峰值如何沿着有胚中胚层移动的数学公式。(C)2009爱思唯尔有限公司保留所有权利。
In vertebrate somitogenesis, "segmentation clock" genes (such as her in zebrafish, hairy in chick, and hes in Mouse) show oscillation, synchronized over nearby cells through cell-cell interaction. The locations of high gene expression appear with regular intervals and move like a wave from posterior to anterior with the speed slowing down toward the anterior end. We analyze traveling wave pattern of her gene expression when there is an anterior-posterior gradient of one of the reaction rates in the gene-protein kinetics. We adopt a model which includes the kinetics of mRNA and proteins of her gene in each cell and cell-cell interaction by Delta-Notch system explicitly. We show that the observed spatio-temporal pattern can be explained if mRNA degradation, protein translation, protein transportation to nucleus occurs faster, or mRNA transcription, Delta protein synthesis occurs slower in posterior than in anterior regions. All of these gradients are those that produce longer periodicity of oscillation of clock gene expression in the anterior than in the posterior. Based on this result, we derive a mathematical formula for how the peak of gene expression moves along the pere-somitic mesoderm. (C) 2009 Elsevier Ltd. All rights reserved.