Influence of donor age on development of gonadal tissue from pouch young of the tammar wallaby, Macropus eugenii, after cryopreservation and xenografting into mice.
Influence of donor age on development of gonadal tissue from pouch young of the tammar wallaby, Macropus eugenii, after cryopreservation and xenografting into mice.
复制标题
冷冻保存并异种移植到小鼠体内后，供体年龄对塔玛袋鼠（Macropus eugenii）幼崽性腺组织发育的影响。
作者：
D. Mattiske;G. Shaw;J. Shaw
将袋鼠(Macropus Eugenii)的卵巢移植到已知发育阶段的真核受者体内，以确定是否会发生正常发育。在减数分裂开始之前，从20天龄的小袋中移植的卵巢保留了很少的生殖细胞，并形成了类似于生精索的管状结构。从50日龄的幼囊发育而来的卵巢含有原始卵泡，在真核宿主内发育成有腔卵泡和黄体，并产生刺激宿主生殖道的激素。与卵巢内原位事件的发生时间相比，卵巢腔形成的开始时间和卵泡发育的进程提前。来自50日龄供体的冻融卵巢发育为腔前卵泡，但发育速度和数量都有所减少。这一发现表明，有袋类物种的性腺可以作为异种移植物在真兽体内发育，形成大的有腔卵泡。异种移植的卵泡发育加速为研究控制卵泡发育的因素提供了一个潜在的有价值的模型。利用幼年袋鼠的毛囊在真兽类宿主中作为异种移植，辅助繁殖濒危有袋类动物也是可行的。
Ovaries from a marsupial, the tammar wallaby (Macropus eugenii), were grafted into a eutherian recipient at known stages of development to ascertain whether normal development would occur. Xenografted ovaries from pouch young < 20 days old, before the onset of meiosis, retained few germ cells and developed tubule-like structures reminiscent of seminiferous cords. Ovaries from 50-day-old pouch young, which contain primordial follicles, developed into antral follicles and corpora lutea within the eutherian host, and produced hormones that stimulated the reproductive tract of the host. The timing of onset of antrum formation and the progress of follicle development were advanced relative to the timing of events in ovaries in situ. Frozen-thawed ovaries from 50-day-old donors developed into preantral follicles, but at a reduced rate and number. This finding shows that gonads of a marsupial species can develop as xenografts in a eutherian, forming large antral follicles. Accelerated follicular development in xenografts provides a potentially valuable model for studying the factors that control follicle development. Assisted reproduction of endangered marsupials may also be feasible using follicles from pouch young grown as xenografts in a eutherian host.
影响因子：
4.8
作者：
LARA, HE;DEES, WL;OJEDA, SR
通讯作者：
OJEDA, SR