Effects of different diets used in diet-induced obesity models on insulin resistance and vascular dysfunction in C57BL/6 mice
Effects of different diets used in diet-induced obesity models on insulin resistance and vascular dysfunction in C57BL/6 mice
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DOI：
10.1038/s41598-019-55987-x
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发表时间：
2019-12-20
影响因子：
4.6
通讯作者：
Xia, Ning
中科院分区：
文献类型：
--
作者：
Lang, Philipp;Hasselwander, Solveig;Xia, Ning
本研究的目的是比较不同的饮食方式导致肥胖的方式，重点是胰岛素抵抗和血管功能障碍。雄性C57BL/6J小鼠从6周龄开始，分别饲喂标准饲料(SCD)、普通脂肪饲料(NFD)、自助饲料(CAF)和高脂饲料(HFD)，共12周。CAF和HFD都会导致肥胖(体重分别增加179%和194%)、葡萄糖耐受和胰岛素抵抗，程度相当。在含有血管周围脂肪组织(PVAT)的动脉中，乙酰胆碱诱导的血管扩张在NFD组最好，在CAF组最差。CAF组和HFD组均可观察到丝氨酸1177位内皮型一氧化氮合酶的磷酸化水平降低。血浆凝血活性以HFD组最高，SCD组最低。NFD组的凝血活性也明显高于SCD组。综上所述，CAF和HFD都是导致内脏肥胖、糖耐量减低和胰岛素抵抗的可靠的小鼠饮食。在导致PVAT功能障碍和血管功能障碍方面，CAF比HFD更有效，而高凝状态在HFD组最为明显。NFD组凝血活性高于NCD组。
The aim of the present study was to compare different diets used to induce obesity in a head-to-head manner with a focus on insulin resistance and vascular dysfunction. Male C57BL/6J mice were put on standard chow diet (SCD), normal-fat diet (NFD), cafeteria diet (CAF) or high-fat diet (HFD) for 12 weeks starting at the age of 6 weeks. Both CAF and HFD led to obesity (weight gain of 179% and 194%, respectively), glucose intolerance and insulin resistance to a comparable extent. In aortas containing perivascular adipose tissue (PVAT), acetylcholine-induced vasodilation was best in the NFD group and worst in the CAF group. Reduced phosphorylation of endothelial nitric oxide synthase at serine 1177 was observed in both CAF and HFD groups. Plasma coagulation activity was highest in the HFD group and lowest in the SCD group. Even the NFD group had significantly higher coagulation activity than the SCD group. In conclusions, CAF and HFD are both reliable mouse diets in inducing visceral obesity, glucose intolerance and insulin resistance. CAF is more effective than HFD in causing PVAT dysfunction and vascular dysfunction, whereas hypercoagulability was mostly evident in the HFD group. Coagulation activity was higher in NFD than NCD group.