Prostaglandins stimulate the stress‐induced synthesis of hsp27 and αB crystallin
Prostaglandins stimulate the stress‐induced synthesis of hsp27 and αB crystallin
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前列腺素刺激应激诱导的 hsp27 和 αB 晶状体蛋白合成
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Kanefusa Kato
中科院分区：
文献类型：
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作者：
Hidenori Ito;K. Okamoto;Kanefusa Kato
研究了各种前列腺素（前列腺素A1、A2、J2、E2和D2）对C6大鼠胶质瘤细胞中应激诱导的hsp 27和α B晶体蛋白蓄积的影响。通过特异性免疫测定法测定的hsp 27和α B晶体蛋白水平在融合培养的细胞中较低。在细胞暴露于热（42 ° C，30分钟）或亚砷酸盐（50 μ M，1小时）后，这两种蛋白质的水平增加。将细胞暴露于10 μ M前列腺素A1、A2或J2各1 h，导致刺激与热休克转录因子（HSF）的热休克元件（HSE）的结合。然而，HSF 1没有磷酸化依赖的迁移率变化，hsp 27、α B晶体蛋白和hsp 70的转录和翻译也没有随后的增加。当细胞在10 - 40 μ M前列腺素存在下暴露于亚砷酸盐时，hsp 27和α B晶体蛋白在细胞中的积聚显著增强。在前列腺素存在的情况下，用亚砷酸盐处理的细胞中的hsp70水平也增加，如通过Western印迹分析估计的。北方印迹分析显示，在每种前列腺素存在的情况下暴露于亚砷酸盐的细胞中，hsp 27、α B晶状体蛋白和hsp 70的信使RNA（mRNA）表达增强。在热诱导hsp 27、α B晶状体蛋白和hsp 70反应的情况下，也观察到了类似的胡枝子素刺激作用。凝胶迁移率变动分析显示，每种前列腺素延长了亚砷酸盐诱导的HSF与HSE的结合。这些结果表明，药理剂量的野牡丹素通过激活HSF刺激应激诱导的应激蛋白合成。J.细胞。170：255 - 262，1997。© 1997 Wiley利斯公司
The effects were examined of various prostaglandins (prostaglandin A1, A2, J2, E2, and D2) on the stress‐induced accumulation of hsp27 and αB crystallin in C6 rat glioma cells. The levels of hsp27 and αB crystallin, which were determined by specific immunoassays, were low in cells in confluent cultures. The levels of the two proteins increased after exposure of cells to heat (42°C for 30 min) or arsenite (50 μM for 1 h). Cells exposed to 10 μM each of prostaglandin A1, A2, or J2 for 1 h resulted in stimulation of the binding to the heat shock element (HSE) of heat shock transcription factor (HSF). However, there was no phosphorylation‐dependent mobility shift of HSF1 and no subsequent increase in the transcription and translation for hsp27, αB crystallin, and hsp70. When cells were exposed to arsenite in the presence of 10‐40 μM prostaglandin, the accumulation of hsp27 and αB crystallin in cells was enhanced markedly. The levels of hsp70 also increased in cells that had been treated with arsenite in the presence of a prostaglandin, as estimated by Western blot analysis. Northern blot analysis revealed that the expression of messenger RNAs (mRNAs) for hsp27, αB crystallin, and hsp70 was enhanced in cells that had been exposed to arsenite in the presence of each prostaglandin. Similar stimulatory effects of prostaglandins also were observed in the case of the heat‐induced responses of hsp27, αB crystallin, and hsp70. Gel mobility shift assays revealed that each prostaglandin prolonged the arsenite‐induced binding of HSF to HSE. These results suggest that the pharmacological dose of prostaglandins stimulates the stress‐induced synthesis of stress proteins via activation of the HSF. J. Cell. Physiol. 170:255–262, 1997. © 1997 Wiley‐Liss, Inc.
DOI：
10.1073/pnas.89.14.6227
发表时间：
1992-07-15
影响因子：
11.1
作者：
AMICI, C;SISTONEN, L;MORIMOTO, RI
通讯作者：
MORIMOTO, RI