Brain aromatase expression after experimental stroke: Topography and time course
Brain aromatase expression after experimental stroke: Topography and time course
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DOI：
10.1016/j.jsbmb.2005.02.016
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发表时间：
2005-06-01
影响因子：
4.1
通讯作者：
Macrae, IM
中科院分区：
文献类型：
--
作者：
Carswell, HVO;Dominiczak, AF;Macrae, IM
脑芳香化酶在神经毒性损伤后表达增加，并通过产生局部雌激素发挥神经保护作用。本研究探讨了实验性脑卒中（大脑中动脉闭塞（MCAO））后脑芳香化酶表达的地形图和时程。切除卵巢的易卒中自发性高血压大鼠通过电凝进行远端MCAO。免疫组织化学显示，增加大脑芳香化酶表达在24小时和8天的皮质半影区/梗死周围区，没有增加明显的2小时或30天后MCAO。双标记研究表明，一些增加的芳香化酶表达与星形胶质细胞的过程。因此，这是芳香酶蛋白在MCAO后增加的第一个证据，并且位置（梗塞周围）、时间过程（24小时内）和细胞定位（星形细胞）表明芳香酶通过雌激素的局部合成促进实验性中风后半影区中细胞存活的潜力。(c)2005爱思唯尔有限公司保留所有权利。
Brain aromatase has been shown to be increased in expression after neurotoxic damage and to exert neuroprotection via generation of local oestrogens. The present study investigates the topography and time course of brain aromatase expression after experimental stroke (middle cerebral artery occlusion (MCAO)). Ovariectomised stroke prone spontaneously hypertensive rats underwent distal MCAO by electrocoagulation. Immunohistochemistry revealed increased brain aromatase expression at 24 h and 8 days in the cortical penumbra/periinfarct zones with no increase evident at 2h or 30 days post-MCAO. Double label studies indicate that some of the increased aromatase expression is associated with astrocytic processes. Thus, this is the first evidence that aromatase protein is increased after MCAO and the location (peri-infarct), time course (within 24h) and cellular localisation (astrocytic) indicate the potential for aromatase to promote the survival of cells in the penumbra after experimental stroke by local synthesis of oestrogens. (c) 2005 Elsevier Ltd. All rights reserved.