H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division
H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division
复制标题
H–NS、IHF 和 DnaA 导致类核组织、复制起始和细胞分裂的变化
DOI：
10.1002/jobm.201900497
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发表时间：
2020-02-01
影响因子：
3.1
通讯作者：
Moregen, Morigen
中科院分区：
文献类型：
--
作者：
Huang, Tingting;Yuan, Hong;Moregen, Morigen
组蛋白样核结构蛋白(H-NS)和整合宿主因子(IHF)是主要的类核相关蛋白，复制启动子DNAA也可能与类核致密有关。已有研究表明，依赖于蛋白质的DNA紧凑与细菌生理的许多方面有关，包括转录、DNA复制和位点特异性重组。然而，类核致密导致细菌生理学的机制仍不清楚。在这里，我们证明了H-NS对于以一种介质无关的方式进行正确的类核压缩是重要的。H-NS介导的类核紧凑不是细胞正确分裂所必需的，但后者依赖于富含H-NS的培养基中的H-NS。此外，研究还发现，IHFα介导的类核紧凑是正确的细胞分裂所必需的，并且这种作用依赖于介质。此外，我们还证明了H-NS和IHF对类核紧凑的影响是累积的。有趣的是，DNAA在类核紧凑中也起着重要的作用，并且DNAA对类核紧凑的影响似乎与细胞分裂以一种介质依赖的方式有关。这些结果表明，复制的混乱启动、不正确的细胞分裂和缓慢的生长很可能直接或间接地与类核组织的紊乱有关。
Histone-like nucleoid-structuring protein (H-NS) and integration host factor (IHF) are major nucleoid-associated proteins, and DnaA, a replication initiator, may also be related with nucleoid compaction. It has been shown that protein-dependent DNA compaction is related with many aspects of bacterial physiology, including transcription, DNA replication, and site-speci?c recombination. However, the mechanism of bacterial physiology resulting from nucleoid compaction remains unknown. Here, we show that H-NS is important for correct nucleoid compaction in a medium-independent manner. H-NS-mediated nucleoid compaction is not required for correct cell division, but the latter is dependent on H-NS in rich medium. Further, it is found that the IHF alpha-mediated nucleoid compaction is needed for correct cell division, and the effect is dependent on medium. Also, we show that the effects of H-NS and IHF on nucleoid compaction are cumulative. Interestingly, DnaA also plays an important role in nucleoid compaction, and the effect of DnaA on nucleoid compaction appears to be related to cell division in a medium-dependent manner. The results presented here suggest that scrambled initiation of replication, improper cell division, and slow growth is likely associated with disturbances in nucleoid organization directly or indirectly.