Cell volume and metabolic dependence of NEM-activated K+-Cl- flux in human red blood cells.
Cell volume and metabolic dependence of NEM-activated K+-Cl- flux in human red blood cells.
复制标题
人红细胞中 NEM 激活的 K -Cl - 通量的细胞体积和代谢依赖性。
DOI：
10.1152/ajpcell.1985.249.1.c124
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Adragna,NC
中科院分区：
文献类型：
--
作者：
Lauf,PK;Perkins,CM;Adragna,NC
用Rb+作为K+类似物，在人红细胞中研究了异渗培养基孵育和代谢耗竭对n-乙基马来酰亚胺（NEM）刺激的耐瓦阿巴因（OR） Cl依赖性K+流入的影响。在磷酸盐缓冲的各渗介质中，nem刺激的而不是基础的Rb+- cl -内流被发现是细胞体积依赖的。当细胞ATP （[ATP]c）通过暴露于不可代谢的2-脱氧-d -葡萄糖而降低到其初始水平的0.10以下时，nem刺激的Rb+流入被消除，但基础Cl-依赖性Rb+流入未被消除。代谢耗竭的红细胞随后通过葡萄糖加肌苷孵育恢复了nem诱导的Rb+ (K+)运输活性。ATP分解和Rb+内流抑制在时间上的差异表明，代谢激活nem刺激的Rb+通量可能涉及ATP以外的因素。
The effects of incubation in anisosmotic media and of metabolic depletion on ouabain-resistant (OR) Cl--dependent K+ influxes stimulated by N-ethylmaleimide (NEM) were studied in human red blood cells using Rb+ as K+ analogue. The NEM-stimulated but not the basal Rb+-Cl- influx measured in phosphate-buffered anisosmotic media was found to be cell volume dependent. When cellular ATP, [ATP]c, was lowered to less than 0.10 of its initial level by exposure to nonmetabolizable 2-deoxy-D-glucose, the NEM-stimulated but not the basal Cl--dependent Rb+ influxes were abolished. Metabolically depleted red blood cells subsequently repleted by incubation in glucose plus inosine regained the NEM-inducible Rb+ (K+) transport activity. The difference in the time course of ATP breakdown and Rb+ influx inhibition suggests that energization of the NEM-stimulated Rb+ flux by metabolism may involve factors additional to ATP.