THE LONG‐TERM CULTURE OF A DIPLOID RAT HEPATOCYTE CELL STRAIN *
THE LONG‐TERM CULTURE OF A DIPLOID RAT HEPATOCYTE CELL STRAIN *
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二倍体大鼠肝细胞株的长期培养 *
DOI：
10.1111/j.1749-6632.1980.tb29524.x
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发表时间：
1980
影响因子：
5.2
通讯作者：
W. Schaeffer
中科院分区：
文献类型：
--
作者：
W. Schaeffer
我们建立了一种克隆大鼠肝细胞的细胞培养体系RL-PR-C。该培养物已在培养物中生长超过150代（560 p.d.）。通过众数计数，它保持二倍体超过50代（183 p.d.）之后，在41和42之间存在模态数的变化。大约在这个时候，培养物变得内在地（自发地）恶性转化，而用黄曲霉毒素B1以亚急性和亚毒性剂量处理的细胞从第12代（65 p.d.）在第35代（135 p.d.）发生恶性转化。有证据表明，并不是群体中的所有细胞都是恶性转化的。将恶性转化细胞的混合群体回注射到同基因大鼠幼仔中，产生组织学分类为混合的肿瘤。也就是说，同一组织切片的区域表现出癌和肉瘤的特征。通过注射来源于恶性转化细胞的克隆获得的数据表明，注射的细胞相对于形成的肿瘤类型是多能的。这方面的进一步调查仍在继续。该培养系统将用于研究转化状态的演变和维持，并开发推定致癌物和诱变剂的试验系统。
We have developed a cell culture system of cloned rat hepatocytes which is named RL-PR-C. This culture has been grown in culture for over 150 passages (560 p.d.). It remains diploid by modal number count for over 50 passages (183 p.d.) after which there is a variation in the modal number between 41 and 42. The culture becomes intrinsically (spontaneously) malignantly transformed at about this time whereas cells treated with aflatoxin B1, at subacute and subtoxic doses, from Passage 12(65 p.d.) were malignantly transformed at Passage 35(135 p.d.). Evidence indicates that not all cells in the population are malignantly transformed. Back injection of mixed population of malignantly transformed cells into isogenic rat pups, yield tumors which are histologically classified as mixed. That is, areas of the same tissue slice exhibit both carcinomas and sarcoma characteristics. Data obtained by injection of clones derived from malignantly transformed cells suggest that the cells injected are multipotent with respect to the type of tumor formed. Further investigations are continuing in this regard. This cultural system will be used to study the evolution and maintenance of the transformed state and to develop a test system for putative carcinogens and mutagens.