Effect of tissue fluids on hydrophobicity and adherence of Enterococcus faecalis to dentin
Effect of tissue fluids on hydrophobicity and adherence of Enterococcus faecalis to dentin
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DOI：
10.1016/j.joen.2007.07.035
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发表时间：
2007-12-01
影响因子：
4.2
通讯作者：
Kishen, Anil
中科院分区：
文献类型：
--
作者：
George, Saji;Kishen, Anil
进行该体外研究以确定（1）所选口腔细菌的疏水性，（2）生长培养基（唾液和血清）和生长模式（促渗剂或生物膜）对粪肠球菌的疏水性的影响，以及（3）生长培养基和调节液对E.粪便到牙本质。与烃相（二甲苯）结合的能力用作细胞相对疏水性的指标。应用荧光显微镜技术检测牙本质表面细菌粘附情况。结果表明，参与口腔生物膜形成初期的细菌如变形链球菌、具核梭杆菌和牙龈卟啉单胞菌的疏水性比E.粪便E.在饥饿和生物膜生长模式下，粪菌数量显著增加（p
This in vitro study was carried out to determine (1) the hydrophobicity of selected oral bacteria, (2) the influence of growth media (saliva and serum) and mode of growth (planktonic or biofilm) on the hydrophobicity of Enterococcus faecalis, and (3) the influence of growth media and conditioning fluids on the adherence of E. faecalis to dentin. The ability to bind to a hydrocarbon phase (xylene) was used as an index of relative hydrophobicity of cells. Fluorescent microscopy-based technique was used to assay the bacterial adherence to dentin. Results showed that bacteria involved in the primary stage of oral biofilm formation such as Streptococcus mutans, Fusobacterium nucleatum, and Porphyromonas gingivalis are relatively more hydrophobic than E. faecalis. The hydrophobicity of E. faecalis was significantly increased during starvation and biofilm mode of growth (p