Sleep/wake dependent changes in cortical glucose concentrations
Sleep/wake dependent changes in cortical glucose concentrations
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DOI：
10.1111/jnc.12063
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发表时间：
2013-01-01
影响因子：
4.7
通讯作者：
Cirelli, Chiara
中科院分区：
文献类型：
--
作者：
Dash, Michael B.;Bellesi, Michele;Cirelli, Chiara
大脑中的大部分能量来自葡萄糖，并支持谷氨酸能活动。皮质谷氨酸能神经元的放电率以及皮质细胞外谷氨酸水平随着清醒时间的延长而增加，并在整个非快速眼动睡眠过程中下降，这引发了血糖水平是否反映行为状态和睡眠/觉醒历史的问题。在这里，大鼠大脑皮层的慢性(23天)脑电记录与固定电位安培法相结合，在自然睡眠-觉醒周期和睡眠剥夺3小时的反应中，以秒为基础监测细胞外葡萄糖浓度([Gluc])。[Gluc]在非快速眼动睡眠中逐渐升高，在快速眼动睡眠中逐渐下降，而在觉醒期间，[Gluc]早期下降，然后在醒来后815分钟上升。在大鼠大部分处于清醒状态的黑暗阶段，存在显著的白天效应，夜间最后34h的[Gluc]明显低于前34h。此外，清醒期间[Gluc]下降的早期持续时间长于巩固睡眠后。因此，睡眠/清醒史可能会影响清醒时大脑可利用的葡萄糖水平。
Most of the energy in the brain comes from glucose and supports glutamatergic activity. The firing rate of cortical glutamatergic neurons, as well as cortical extracellular glutamate levels, increase with time spent awake and decline throughout non rapid eye movement sleep, raising the question whether glucose levels reflect behavioral state and sleep/wake history. Here chronic (23 days) electroencephalographic recordings in the rat cerebral cortex were coupled with fixed-potential amperometry to monitor the extracellular concentration of glucose ([gluc]) on a second-by-second basis across the spontaneous sleep-wake cycle and in response to 3 h of sleep deprivation. [Gluc] progressively increased during non rapid eye movement sleep and declined during rapid eye movement sleep, while during wake an early decline in [gluc] was followed by an increase 815 min after awakening. There was a significant time of day effect during the dark phase, when rats are mostly awake, with [gluc] being significantly lower during the last 34 h of the night relative to the first 34 h. Moreover, the duration of the early phase of [gluc] decline during wake was longer after prolonged wake than after consolidated sleep. Thus, the sleep/wake history may affect the levels of glucose available to the brain upon awakening.