Structures of silk fibroin before and after spinning and biomedical applications
Structures of silk fibroin before and after spinning and biomedical applications
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丝素蛋白纺丝前后的结构及生物医学应用
DOI：
10.1038/pj.2016.77
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发表时间：
2016
期刊：
影响因子：
2.8
通讯作者：
Suzuki Y.
中科院分区：
文献类型：
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作者：
天野吉貴，宮瑾;古川英光;Masanori Arai ; Jin Gong ; Masato Makino ; M. Hasnat Kabir ; Hidemitsu Furukawa;新井正徳，熊谷大慧，宮瑾，牧野真人，川上勝，古川英光;宮瑾;Suzuki Y.
蚕通过在环境温度下在丝素蛋白水溶液中施加剪切应力来生产丝素蛋白纤维。该工艺是开发可持续纤维加工技术的有吸引力的模式。然而，为了完全阐明丝素蛋白的加工机理，需要确定纺丝前后丝素蛋白的结构。在这项研究中，我们报告了纺丝前后丝素蛋白的结构，通过溶液和固态核磁共振（NMR）确定。利用从家蚕幼虫中提取的天然液体丝，通过溶液NMR确定丝素串联重复序列的纺丝前结构。此外，通过结合稳定同位素标记的模型肽和固态核磁共振研究纺丝后的丝素蛋白的精确层状结构。此外，通过静电纺丝开发了基于丝的小直径血管移植物，并随后进行了体内评价。这些研究为节能纤维加工技术和蚕丝基生物医用材料的研究提供了一个新的视角。
Silkworms produce silk fibroin fibers from an aqueous silk fibroin solution by applying shear stress within the spinneret at ambient temperature. This process is an attractive model for developing sustainable fiber processing technology. However, to completely elucidate the fibroin processing mechanism, the structures of fibroin before and after spinning need to be determined. In this study, we report the structures of silk fibroin before and after spinning, determined by solution and solid-state nuclear magnetic resonance (NMR). The pre-spinning structure of fibroin tandem repeat sequences was determined by solution NMR, using native liquid silk extracted from silkworm larvae. In addition, the precise lamellar structure of fibroin after spinning was investigated through a combination of stable isotope labeling of model peptides and solid-state NMR. Moreover, a silk-based small diameter vascular graft was developed by electrospinning and was subsequently evaluated in vivo. These studies may provide a perspective for investigation of energy-conserving fiber processing techniques and silk-based biomedical materials.