Modulation of angiogenic functions in human macrophages by biomaterials
Modulation of angiogenic functions in human macrophages by biomaterials
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DOI：
10.1016/s0142-9612(03)00201-1
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发表时间：
2003-09-01
期刊：
影响因子：
14
通讯作者：
Zwadlo-Klarwasser, G
中科院分区：
文献类型：
--
作者：
Dagtekin, G;Schiffer, R;Zwadlo-Klarwasser, G
我们通过测量编码血管生成和抗血管生成分子（包括碱性成纤维细胞生长因子（bFGF）、血管内皮生长因子（VEGF）、血管生成素-1（Ang-1）和血小板反应蛋白-1（Tsp-1））的基因的mRNA表达，研究了聚氯乙烯（PVC）、聚四氟乙烯（PTFE）和组织培养聚苯乙烯（TCPS）影响人单核细胞来源的巨噬细胞血管生成功能的能力。通过内皮细胞的增殖和人胎盘血管碎片中新毛细血管的萌发来测量相应巨噬细胞条件培养基（CM）的血管生成活性。我们确定 bFGF 在巨噬细胞中不表达，而 VEGF 和 Tsp-1 mRNA 则组成型表达。 Ang-1 在 PTFE 和 TCPS 上培养长达 7 天的巨噬细胞中表达，与培养阶段无关。相比之下，在 PVC 上培养的巨噬细胞在 7 天后没有产生可检测量的 Ang-1 mRNA。来自在 PTFE 或 TCPS 上培养的巨噬细胞的 CM 刺激血管生成，而来自在 PVC 上培养的巨噬细胞的 CM 抑制血管生成。结果表明，聚合物可以引起巨噬细胞中血管生成分子Ang-1的差异表达。它们还诱导巨噬细胞的不同表型，这些巨噬细胞可以刺激或抑制血管生成，这表明对新血管形成的影响具有材料依赖性。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
We examined the ability of polyvinylchloride (PVC), polytetrafluorethylene (PTFE) and tissue culture polystyrene (TCPS) to affect angiogenic functions in human monocyte-derived macrophages by measuring the mRNA expression of genes encoding angiogenic and anti-angiogenic molecules including basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1) and thrombospondin-1 (Tsp-1). The angiogenic activity of the corresponding macrophage conditioned media (CM) was measured by the proliferation of endothelial cells and the sprouting of new capillaries from fragments of human placental blood vessels. We determined that bFGF was not expressed in macrophages while VEGF and Tsp-1 mRNAs were expressed constitutively. Ang-1 was expressed in macrophages cultured up to 7 days on PTFE and TCPS independent of the culture stage. In contrast, macrophages cultured on PVC did not produce detectable amounts of Ang-1 mRNA after 7 days. CM from macrophages cultured either on PTFE or TCPS stimulated angiogenesis whereas CM from macrophages cultured on PVC inhibited it. The results demonstrate that polymers can cause differential expression of the angiogenic molecule Ang-1 in macrophages. They also induce different phenotypes of macrophages, which can either stimulate or inhibit angiogenesis suggesting a material-dependent influence on neovascularization. (C) 2003 Elsevier Science Ltd. All rights reserved.