Single-molecule cut-and-paste surface assembly
Single-molecule cut-and-paste surface assembly
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DOI：
10.1126/science.1151424
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发表时间：
2008-02-01
期刊：
影响因子：
56.9
通讯作者：
Gaub, H. E.
中科院分区：
文献类型：
--
作者：
Kufer, S. K.;Puchner, E. M.;Gaub, H. E.
我们介绍了一种自下而上组装生物分子结构的方法，该方法结合了原子力显微镜（AFM）的精度和DNA杂交的选择性。通过与AFM针尖结合的互补DNA链从储库区域拾取与DNA寡聚体偶联的功能单元。这些单元被转移并沉积在目标区域上，以创建基本的几何结构，由具有不同功能的单元组装而成。每一个剪切和粘贴事件的特征在于单分子力光谱和单分子荧光显微镜。传输和沉积超过5000个单位，传输效率损失小于10%。
We introduce a method for the bottom- up assembly of biomolecular structures that combines the precision of the atomic force microscope ( AFM) with the selectivity of DNA hybridization. Functional units coupled to DNA oligomers were picked up from a depot area by means of a complementary DNA strand bound to an AFM tip. These units were transferred to and deposited on a target area to create basic geometrical structures, assembled from units with different functions. Each of these cut- and- paste events was characterized by single- molecule force spectroscopy and single- molecule fluorescence microscopy. Transport and deposition of more than 5000 units were achieved, with less than 10% loss in transfer efficiency.