Elevated TERT Expression in TERT-Wildtype Adult Diffuse Gliomas: Histological Evaluation with a Novel TERT-Specific Antibody.
Elevated TERT Expression in TERT-Wildtype Adult Diffuse Gliomas: Histological Evaluation with a Novel TERT-Specific Antibody.
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DOI：
10.1155/2018/7945845
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发表时间：
2018
影响因子：
--
通讯作者：
Shibata N
中科院分区：
文献类型：
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作者：
Masui K;Komori T;Kato Y;Masutomi K;Ichimura K;Ogasawara S;Kaneko MK;Oki H;Suzuki H;Nitta M;Maruyama T;Muragaki Y;Kawamata T;Sawada T;Shibata N
端粒酶逆转录酶（TERT）在弥漫性胶质瘤的生物学研究中具有重要意义。TERT启动子突变选择性地在1p/19q编码缺失的少突胶质细胞瘤和异柠檬酸脱氢酶基因- (IDH-)野生型胶质母细胞瘤（GBM）中观察到。然而，TERT转录物在包括胶质瘤在内的各种癌症中分布广泛，迄今为止很少研究TERT蛋白的表达。因此，除了TERT的突变状态外，检测TERT在肿瘤中的表达水平是至关重要的，迫切需要检测TERT蛋白表达的敏感和特异性的方法来评估胶质瘤中TERT的生物学特性。使用我们新开发的适用于人体组织的TERT特异性单克隆抗体（TMab-6），我们发现TERT野生型、TERT突变胶质瘤和肿瘤血管中TERT表达意外增加。这是首次对人胶质瘤组织中TERT免疫反应性表达的广泛分析，提示除了启动子突变外，TERT蛋白表达可能受多种机制的调节。
Telomerase reverse transcriptase (TERT) is important for the biology of diffuse gliomas. TERT promoter mutations are selectively observed among 1p/19q-codeleted oligodendrogliomas and isocitrate dehydrogenase gene- (IDH-) wildtype glioblastoma (GBM). However, TERT transcripts range widely in various cancers including gliomas, and TERT protein expression has been rarely investigated thus far. It would be thus critical to examine the expression level of TERT in tumors in addition to its mutational status, and sensitive and specific methods are urgently needed to examine TERT protein expression for the assessment of TERT biology in gliomas. Using our newly developed TERT-specific monoclonal antibody (TMab-6) applicable to human tissue, we found an unexpected increase in TERT expression in TERT-wildtype as well as TERT-mutated gliomas and in tumor vasculature. This is the first extensive analysis on the expression of TERT immunoreactivity in human glioma tissue, suggesting that TERT protein expression may be regulated by several mechanisms in addition to its promoter mutation.
DOI：
10.1089/mab.2016.0037
发表时间：
2016-10
影响因子：
--
作者：
Ogasawara S;Fujii Y;Kaneko MK;Oki H;Sabit H;Nakada M;Suzuki H;Ichimura K;Komori T;Kato Y
通讯作者：
Kato Y