Qualitative and quantitative freeze-fracture studies on olfactory and nasal respiratory structures of frog, ox, rat, and dog
Qualitative and quantitative freeze-fracture studies on olfactory and nasal respiratory structures of frog, ox, rat, and dog
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青蛙、牛、大鼠和狗嗅觉和鼻呼吸结构的定性和定量冷冻断裂研究
DOI：
10.1007/bf00237805
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发表时间：
1980
影响因子：
3.6
通讯作者：
Dr. B. Ph. M. Menco
中科院分区：
文献类型：
--
作者：
Dr. B. Ph. M. Menco
本实验采用冷冻断裂法对蛙、牛、大鼠和狗的嗅和鼻呼吸道上皮细胞的表面结构进行了比较研究。特别注意纤毛和微绒毛存在于这些表面，虽然观察包括各种其他结构，如小的细胞内空泡存在于嗅觉受体末梢和罕见的刷细胞。在覆盖在嗅上皮上的粘液中，可以看到膜泡，通常附着在嗅纤毛上。其中一些显示膜内颗粒分布类似于其余的纤毛，而另一些则没有颗粒。光滑囊泡也存在于其他类型上皮（呼吸道上皮和Bowman腺）的粘液中。冷冻断裂形态的细胞内分泌空泡存在于嗅支持，鲍曼氏和呼吸腺细胞的青蛙是相似的，在所有这些上皮细胞。定量比较的不同结构的利益。当校正纤毛没有观察到，哺乳动物受体末梢承担17纤毛平均，而青蛙受体末梢有6纤毛。纤毛和微绒毛的直径的相对大小是，除了青蛙，相同的所有物种的研究。其他结构的尺寸，例如，比较了不同物种的轴突、树枝状突起和树枝状末端。冷冻裂缝的直径通常大于使用脱水技术所观察到的直径。树突末梢密度范围为4.5 × 106（青蛙）至8.3 × 106（狗）末梢/cm 2。仅发现这些密度和树突末端直径可能存在性别依赖性差异。
SummaryA comparative study using freeze-fracturing has been made of surface structures of olfactory and nasal respiratory epithelia of frog, ox, rat and dog. Special attention has been paid to cilia and microvilli present at these surfaces, although the observations include various other structures such as small intracellular vacuoles present in the olfactory receptor endings and infrequent brush cells. Within the mucus overlying the olfactory epithelium membranous vesicles, often attached to olfactory cilia, are seen. Some of these show intramembranous particle distributions similar to those of the rest of the cilia, whereas others are devoid of particles. Smooth vesicles are also found in the mucus of other types of epithelium (respiratory epithelium and Bowman's glands). The freeze-fracture morphology of intracellular secretory vacuoles present in olfactory supporting, Bowman's and respiratory glandular cells of the frog is similar in all these epithelia. Quantitative comparisons are made of the different structures of interest. When corrected for cilia which were not observed, mammalian receptor endings bear 17 cilia on average, whereas frog receptor endings have 6 cilia. The relative magnitudes of the diameters of the cilia and microvilli are, except for frog, the same for all species studied. Dimensions of other structures e.g., axons, dendrites and dendritic endings are compared in the various species. Freeze-fracture diameters are usually larger than those seen by techniques using dehydration. Dendritic ending densities range from 4.5 × 106 (frog) to 8.3 × 106 (dog) endings per cm2. Possible sex-dependent differences are only found for these densities and dendritic ending diameters.