Cysteinyl leukotriene 1 receptor expression in nasal polyps
Cysteinyl leukotriene 1 receptor expression in nasal polyps
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DOI：
10.1177/000348940611500513
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发表时间：
2006-05-01
影响因子：
1.4
通讯作者：
Hussain, Iftikhar
中科院分区：
文献类型：
--
作者：
Chao, Siew-Shuen;Graham, Scott M.;Hussain, Iftikhar
目的：鼻息肉的发病机制，被认为涉及不同因素之间的复杂相互作用，目前尚未完全了解。最近的研究表明，半胱氨酰白三烯（CysLTs）参与鼻息肉的发展。为了进一步了解CysLTs在息肉发病机制中的作用，我们研究了CysLT 1受体在鼻息肉中的表达。方法：研究组包括从20例连续患者内镜下切除的息肉。取4例因Graves眼病行眼眶减压术的患者的筛窦粘膜样本作为对照。用兔抗人抗CysLT 1受体多克隆抗体测定CysLT 1受体的存在。通过光学显微镜（40倍放大率）计数上皮层和间质中有和无CysLT 1受体表达的细胞。结果：在鼻息肉和对照标本中，间质中表达CysLT 1受体的细胞显著多于上皮。息肉间质也比对照组含有更多的CysLT 1受体表达细胞（每平方毫米29 × 10（3）+/- 7 × 10（3）vs 3 × 10（3）+/- 3 × 10（3）细胞; p <0.01）。在息肉的上皮中，CysLT 1受体的表达显著高于对照组（7 x 10（3）+/- 3 x 10（3）vs 0个细胞/平方毫米; p = 0.02）。没有显着差异，在息肉患者和患者之间没有Samter的三联征和asthene.Conclusions：CysLT 1受体的显着上调，我们发现在基质和上皮的鼻息肉表明存在的炎症组分的发病机制中的息肉，并可能解释了白三烯修饰剂在其治疗的疗效。
Objectives: The pathogenesis of nasal polyps, thought to involve complex interactions between different factors, is currently not fully understood. Recent studies have suggested the involvement of cysteinyl leukotrienes (CysLTs) in nasal polyp development. To further understand the role of CysLTs in polyp pathogenesis, we studied the expression of CysLT1 receptors in nasal polyps.Methods: The study group comprised polyps removed endoscopically from 20 consecutive patients. Samples of ethmoid mucosa from 4 patients who underwent orbital decompression for Graves' ophthalmopathy were used as controls. The presence of CysLT1 receptors was determined with a rabbit anti-human anti-CysLT1 receptor polyclonal antibody. Cells with and without CysLT1 receptor expression were counted within the epithelial layer and stroma by means of light microscopy (40x magnification).Results: There were significantly more cells expressing CysLT1 receptors in the stroma than in the epithelium in both nasal polyps and control specimens. The stroma of polyps also contained more CysLT1 receptor-expressing cells than did controls (29 x 10(3) +/- 7 x 10(3) versus 3 x 10(3) +/- 3 x 10(3) cells per square millimeter; p < .01). In the epithelium of polyps, there was significantly higher expression of CysLT1 receptors than in controls (7 x 10(3) +/- 3 x 10(3) versus 0 cells per square millimeter; p = .02). No significant differences in polyps were found between patients with and patients without Samter's triad and asthma.Conclusions: The significant up-regulation of CysLT1 receptors we found in both the stroma and the epithelium of nasal polyps suggests the presence of an inflammatory component in the pathogenesis of polyps, and possibly explains the efficacy of leukotriene modifiers in their treatment.